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Merck
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重要文件

SCT035

Sigma-Aldrich

BioTracker 515 Green ONOO- Dye

Live cell imaging dye for peroxynitrite (ONOO-) used to detect reactive oxygen species (ROS) generated from the interaction of nitric oxide (NO) and superoxide (O).

同義詞:

Live cell imaging probe

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32161000
NACRES:
NA.47
暫時無法取得訂價和供貨情況

技術

cell based assay: suitable

檢測方法

fluorometric

運輸包裝

ambient

一般說明

Peroxynitrite (ONOO-) is a strong oxidant generated from the interaction of nitric oxide (NO) and superoxide (O). The in vivo formation of this compound has recently been demonstrated in macrophages, neutrophils and endothelial cells. The role of ONOO- in inflammatory diseases is further supported by the detection of immunoreactive nitrotyrosine on proteins at sites of inflammation.

The BioTracker 515 Green ONOO- Dye is a fluorescent probe that detects peroxynitrite (ONOO-) in living cells. Fluorescent intensity is not increased by the existence of hydroxyl radical, singlet oxygen, hydrogen peroxide, hypochlorite, nitric oxide or superoxide.

Spectral Properties
Absorbance: 490nm
Emission: 515nm

儲存和穩定性

Store BioTracker 515 Green ONOO- Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Questions

  1. How long is the dye stable and how long can I monitor the dye under the microscope?

    1 answer

    1. The specific retention time of this dye has not been determined. However, the intracellular retention and fluorescent properties may be comparatively more stable when compared to fluorescein due to the nitro BODIPY-based fluorescent moiety present in SCT035.

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