SCR544
人STEMCCA组成型多顺反子（OKSM）慢病毒重编程试剂盒
The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
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About This Item
分類程式碼代碼:
12352207
eCl@ss:
32161000
NACRES:
NA.71
推薦產品
品質等級
製造商／商標名
STEMCCA
技術
cell culture | stem cell: suitable
輸入
sample type induced pluripotent stem cell(s)
運輸包裝
dry ice
一般說明
人STEMCCA组成型多顺反子（OKSM）慢病毒试剂盒包含高滴度多顺反子（OKSM）慢病毒和聚凝胺转染试剂，已经过验证可用于从人包皮成纤维细胞（HFF）生成人诱导多能干细胞。 STEMCCA载体由转录因子人Oct-4、Klf4、SOX-2和c-Myc（OKSM）组成，由自切割2A肽和IRES序列隔开（Sommer CA，2009）。 使用单个多顺反子慢病毒载体可显著提高重编程效率并减少病毒整合的数量。
成分
1. 人STEMCCA组成型（OKSM）慢病毒： （部件号SCR544-1）两（2）瓶EF1α-hSTEMCCA （OKSM）慢病毒（部件号CS204502）。 每瓶包含15 µL高滴度慢病毒。
2. 聚凝胺转染试剂： （部件号TR-1003-50UL）一（1）瓶包含50 µL 10 mg/mL储备液。
2. 聚凝胺转染试剂： （部件号TR-1003-50UL）一（1）瓶包含50 µL 10 mg/mL储备液。
品質
测试以确认从p6人包皮成纤维细胞生成iPS细胞。 尚未测试其他细胞类型，因此无法保证相似的结果。
儲存類別代碼
12 - Non Combustible Liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Yang Yu et al.
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Human pluripotent stem cells, including cloned embryonic and induced pluripotent stem cells, offer a limitless cellular source for regenerative medicine. However, their derivation efficiency is limited, and a large proportion of cells are arrested during reprogramming. In the current study
Frank Griscelli et al.
Oncotarget, 10(28), 2693-2708 (2019-05-21)
Recent development of cell reprogramming technologies brought a major hope for future cell therapy applications by the use of these cells or their derivatives. For this purpose, one of the major requirements is the absence of genomic alterations generating a
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Myocardial infarction (MI) is an increasing medical problem; however, its pathogenesis has yet to be elucidated and more effective treatment strategies are required. Induced pluripotent stem cells (iPSCs) were recently successfully generated using human somatic cells transfected with four transcription
Martin Madill et al.
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Amyotrophic lateral sclerosis, a devastating neurodegenerative disease, is characterized by the progressive loss of motor neurons and the accumulation of misfolded protein aggregates. The latter suggests impaired proteostasis may be a key factor in disease pathogenesis, though the underlying mechanisms
Areechun Sotthibundhu et al.
Stem cell research & therapy, 7(1), 166-166 (2016-11-17)
Cellular reprogramming is a stressful process, which requires cells to engulf somatic features and produce and maintain stemness machineries. Autophagy is a process to degrade unwanted proteins and is required for the derivation of induced pluripotent stem cells (iPSCs). However
文章
Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.
條款
生成人類誘導多能幹細胞 (iPSC) 的幹細胞重編方案，包括以病毒和非病毒 RNA 為基礎的方法。
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
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