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Merck
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SCM304

Sigma-Aldrich

3dGRO® Human Colon Organoid Expansion Medium

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

同義詞:

Colon Organoid Culture Media, Intestinal Organoid Culture Media

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

形狀

liquid

包裝

pkg of 50 mL

技術

cell culture | stem cell: suitable

滲透度

350-375 mOsm

應用

cell analysis

相關類別

一般說明

3dGRO® Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO® Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.

應用

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

Research Category:
  • Cell Culture
  • Stem Cell Research

品質

Appearance (Color): Clear/No Particlulates (Red Liquid)
Osmolality: 350-375 mOsm
pH: 7.0 – 7.4
Sterility Tested: No Growth/Pass
Endotoxin: <2 EU/mL
Mycoplasma: Negative
Functional Assay: Thaw and culture of human colon organoids for 2 passages.

儲存和穩定性

Upon receipt, store at −20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at −20°C until the expiry date.

法律資訊

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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文章

Human epithelial intestinal colonic organoids can be used as an alternative to Caco-2 drug permeability assays for drug screening and compound toxicity testing.

Organoid culture FAQs. Learn how to culture and analyze organoids following established 3D cell culture protocols. Tips and tricks.

器官組織培養常見問題。瞭解如何按照既定的 3D 細胞培養方案培養和分析器官組織。技巧與竅門。

條款

作為 CFTR 功能的快速體外實驗,福斯可林誘導腫脹方案使用人體結腸器官組織,這些器官組織可來自囊腫性纖維化患者組織。

Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.

高特性冷凍保存的人體結腸器官組織,以及從 iPS 細胞分化上皮腸器官組織的分步器官組織培養方案。

A rapid in vitro assay for CFTR function, the forskolin-induced swelling protocol uses human colon organoids, which can be derived from cystic fibrosis patient tissue.

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Explore our portfolio of 3D cell culture tools and technologies for any 3D cell line or application and discover organoids, hydrogels, ULA, microwell, or hydrogel plates, and more.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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