SC1002

Anti-Sox2 Mouse mAb (245610)

lyophilized, clone 245610, Calbiochem^®

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 245610, monoclonal
• 形狀: lyophilized
• 物種活性: human, mouse
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 同型: IgG2a
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Sox2(20674)

描述

一般說明

Protein G purified mouse monoclonal antibody derived by immunizing mice with the specified immunogen and fusing splenocytes with mouse myeloma cells. Recognizes the ~34 kDa Sox2 protein.

Recognizes the ~34 kDa Sox2 protein in NTERA-2 cells.

This Anti-Sox2 Mouse mAb (245610) is validated for use in Immunoblotting, Immunocytochemistry, Flow Cytometry for the detection of Sox2.

免疫原

Human

a recombinant protein consisting of amino acids 135-317 of human Sox2, expressed in E. coli

應用

Immunoblotting (1-2 µg/ml)

Immunocytochemistry (10 µg/ml; see comments)

Flow Cytometry (see comments)

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from 0.2 µm-filter sterilized PBS, 5% trehalose.

重構

Reconstitute with 200 µl sterile PBS for a final stock concentration of 500 µg/ml. Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage. Avoid freeze/thaw cycles of solutions. Thawed aliquots are stable for up to 1 month at 4°C.

分析報告

Positive Control
NTERA-2 cells

其他說明

Avilion, A.A., et al. 2003.Genes Dev.17, 126.
Graham, V., et al. 2003.Neuron39, 749.
Stevanovic, M. 2003.Mol. Biol. Rep.30, 127.
Kishi, M., et al. 2000.Development127, 791.
Uwanogho, D., et al. 1995.Mech. Dev.49, 23.
Yuan, H., et al. 1995.Genes Dev.9, 2635.

For immunocytochemistry, cells should be fixed in PBS containing 4% paraformaldehyde for 20 min, followed by blocking in PBS/10% normal donkey serum/1% BSA/0.1% Triton^™ X-100 detergent for 45 min and staining overnight at 4°C with appropriately diluted primary antibody. For immunoblotting, the best results may be obtained using whole cell or nuclear extracts; Sox2 may be difficult to detect in cytoplasmic extracts. The detection limit for recombinant Sox2 is ~5 ng/lane under reducing and non-reducing conditions. For intracellular staining by flow cytometry, fix cells in 4% paraformaldehyde and permeabilize with 0.1% saponin. Following fixation and permeabilization, dilute the antibody to 50 µg/ml and add 10 µl diluted antibody to 1-2.5 x 10⁵ cells in a total volume of ≤200 µl. Following incubation with appropriate detection antibody, the cells should be washed a final time with 0.1% saponin prior to analysis. Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1