PC635

PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb

liquid, Calbiochem^®

• 同義詞: Anti-Nibrin (pSer³⁴³) Rabbit pAb
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: diluted serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: liquid
• 不包含: preservative
• 物種活性: human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 同型: IgG
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pSer343)
• 基因資訊: human ... NBN(4683)

描述

一般說明

Rabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser³⁴³.

Recognizes the ~95 kDa nibrin protein phosphorylated at Ser³⁴³ in δ-irradiated lymphoblastoid cells.

This PhosphoDetect Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

免疫原

Human

a synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid

應用

ELISA (1:16,000)

Immunoblotting (1:1000)

Immunoprecipitation (1 µl/500 µg cell extract)

警告

Toxicity: Standard Handling (A)

外觀

Serum diluted in PBS.

重構

Following initial thaw, aliquot and freeze (-20°C).

分析報告

Positive Control
γ-irradiated lymphoblastoid cells

其他說明

Gatei, M., et al. 2000. Nat. Genet.25, 115.
Lim, D.S., et al. 2000. Nature404, 613.
Wu, G., et al. 2000. J. Biol. Chem.275, 30618.
Zhong, Q., et al. 1999. Science285, 747.
Carney, J.P., et al. 1998. Cell93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem.273, 21447.
Varon, R., et al. 1998. Cell93, 467.

This antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable