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MRCF0R100

Millipore

Microcon® DNA快速流动离心过滤器

sample volume 0.5 mL, Ultracel® regenerated cellulose membrane (low binding)

同義詞:

Centrifuge concentrator

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About This Item

分類程式碼代碼:
41104916
eCl@ss:
36100101
NACRES:
NB.24
暫時無法取得訂價和供貨情況

材料

Ultracel® regenerated cellulose membrane (low binding)
polyacetal base (membrane support)
polycarbonate device top
polypropylene tube (for filtrate/concentrate)
silicone O-ring (medical-grade)

品質等級

400

無菌

non-sterile

特點

holdup volume> 10 μL

包裝

pkg of 100 ea

製造商／商標名

Microcon®

參數

0.5 mL sample volume

技術

DNA purification: suitable
RNA purification: suitable

長度

4.50 cm (1.8 in.)

直徑

12.3 mm

過濾面積

0.32 cm2

有效容積

0.5 mL

運輸包裝

ambient

相關類別

一般說明

Microcon®离心过滤器提供含水生物样品的高效浓缩、脱盐或缓冲交换，体积范围10–500 μL。Microcon® DNA Fast Flow过滤器对SDS缓冲液基因组DNA的浓缩和回收进行了优化。

應用

Microcon DNA Fast Flow离心过滤装置与超滤膜已被用于提取常规有机DNA，以比较不同提取方法的DNA产量。[1]它也被用于过滤消化后的DNA，进行基因组DNA的电子显微镜分析。[2]

特點和優勢

Microcon®离心过滤器有几个优点：
  • 小体积与反向旋转带来高回收率
  • 低粘合超滤®膜
  • 快速处理
  • 产量浓度系数<20X，具有稳定的重现性

聯結

替代品：42413

法律資訊

MICROCON is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

分析證明 (COA)

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Bacillus spp. Bacteriophage and the Dairy Environment
Adamso KL and McKillip JL
Liaoning Chemical Industry, 23(2), 4-4 (2019)
Matteo Berti et al.
Nature communications, 11(1), 3531-3531 (2020-07-17)
Homologous recombination (HR) factors were recently implicated in DNA replication fork remodeling and protection. While maintaining genome stability, HR-mediated fork remodeling promotes cancer chemoresistance, by as-yet elusive mechanisms. Five HR cofactors - the RAD51 paralogs RAD51B, RAD51C, RAD51D, XRCC2 and

相關內容

Microcon® Centrifugal Filters Applications Guidelines & Specifications

Microcon® Centrifugal Filters simply and efficiently concentrate and desalt solutions of DNA, RNA, protein or other macromolecules, using any centrifuge that can accept 1.5 mL tubes.

Questions

1–3 of 3 Questions  

  1. Can I use a Microcon DNA fast flow device to isolate DNA from a crude cell lysis preparation such a freeze thaw in TE buffer or a chelex 100 extraction?

    1 answer

    1. Yes, a Microcon DNA fast flow device can be used to isolate DNA from a crude cell lysis preparation. However, lysates typically contain heavy particulate contamination, so clarification spins may be necessary to remove particulates before attempting ultrafiltration.

      Helpful?

  2. The user guide indicates you can't autoclave these microcon, but can you sterilize them in a UV Crosslinker?

    1 answer

    1. The Microcon devices are non-sterile. These devices cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol. Please see the protocol for sanitization below:

      1. Reagent Preparation: 70% Ethanol solution (v/v) is prepared by dilution in distilled water. Both the ethanol solution and distilled water used for rinsing should be filter-sterilized using sterile, 0.22 µm sterilizing grade filter device.

      2.Sanitization Protocol:

      A. Fill the Microcon device to its published capacity with filter-sterilized 70% ethanol, and cap the unit (note that the collection tubes can be sanitized in the same manner).

      B. Invert device by hand or in an end-over-end mixer to continuously wet all internal surfaces of the device.

      C. Incubate for a minimum of 5 minutes (note that the device can be capped and left for up to 24 hours if it is not going to be used immediately).

      D. Spin at recommended G-force using appropriate centrifuge until at least 50% of the volume has passed through the device.

      E. Aseptically discard any remaining ethanol from the filter device and filter collection tube.

      F. Immediately rinse away residual ethanol by adding the filter-sterilized water to the device's published capacity, mix gently to wet all areas, and centrifuge until at least 50% of the volume has passed through the device.

      G. Discard any remaining liquid from both the concentrator device and filter collection tube before use.

      DO NOT let the filter membrane dry out in concentrator unit. If the devices are not to be used immediately, fill the devices with filter-sterilized distilled water, cover, and store refrigerated. Remove liquid from device as previously described prior to use.

      Helpful?

  3. Has the color of the Microcon DNA Fast Flow become brighter blue?

    1 answer

    1. Yes, the color of the device has changed. A new blue colorant and clear resin have replaced the original blue reservoir resin, which has been discontinued. Both old and new resin are HP2 Polycarbonate. The functionality of the product has not changed.

      Helpful?

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