MABT887

Anti-PL Scramblase 1 Antibody, clone 9A7

clone 9A7, from mouse

• 同義詞: Phospholipid scramblase 1, PL scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Transplantability-associated protein 1, NOR1, TRA1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 9A7, monoclonal
• 物種活性: mouse
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_035766
• UniProt登錄號: Q9JJ00
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Plscr1(22038)

描述

一般說明

Phospholipid scramblase 1 (UniProt Q9JJ00; also known as Ca(2+)-dependent phospholipid scramblase 1, Nor1, PL scramblase 1, Tra1, Transplantability-associated protein 1) is encoded by the Plscr1 (also known as Tra1b, Tras1) gene (Gene ID 22038) in murine species. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

特異性

Clone 9A7 reacts with murine PL Scramblase 1, and does not cross-reacts with murine PL Scramblase 2/3/4 or human PL Scramblase 1.

免疫原

Epitope: Cytoplasmic domain

Recombinant protein corresponding to the cytoplasmic domain of mouse PL Scramblase 1.

應用

Anti-PL Scramblase 1 Antibody, clone 9A7 is an antibody against PL Scramblase 1 Antibody for use in Western Blotting.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Western Blotting Analysis: A representative lot detected PL Scramblase 1 in mouse lung homogenates. Clone 9A7 also detected LPS treatment-induced PL Scramblase 1 upregulation in liver using total tissue homogenates, as well as cytosolic and nuclear extracts from LPS-treated and untreated mice (Lu, B., et al. (2007). Biochim. Biophys. Acta. 1771(9):1177-1185).

品質

Evaluated by Western Blotting in mouse thymus tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of mouse thymus tissue lysate.

標靶描述

~38 kDa observed. Two additional bands shown correspond to heavy and light chains of endogenous IgG from the mouse thymus tissue.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable