生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3C4, monoclonal
物種活性
goat, mouse, rabbit, human
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1κ
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... HSP90B1(7184)
一般說明
Endoplasmin (UniProt P14625; also known as 94 kDa glucose-regulated protein, Endothelial cell (HBMEC) glycoprotein, Epididymis luminal protein 35, Epididymis secretory sperm binding protein Li 125m, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Stress-inducible tumor rejection antigen gp96, Tumor rejection antigen 1) is encoded by the HSP90B1 (also known as ECGP, GP96, GRP94, HEL35; HEL-S-125m, TRA1) gene (Gene ID 7184) in human. The endoplasmic reticulum (ER) chaperone Grp94 mediates the cell surface export of molecules involved in the native immune response, mesoderm induction, muscle development, cytoprotection against oxidative stress, and calcium homeostasis. Src family tyrosine kinase Fyn-catalyzed Grp94 phosphorylation is reported to be essential for Grp94 export from ER to the Golgi apparatus in the early phase of myoblast differentiation.
免疫原
Epitope: N-terminal half
His-tagged recombinant protein corresponding to the N-terminal of rabbit GRP94.
應用
Anti-GRP94 Antibody, clone 3C4 is an antibody against GRP94 for use in Western Blotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected GRP94 in 10 µg of mouse liver and human heart tissue lysate.
Immunofluorescence Analysis: Representative lots detected GRP94 immunoreactivity in rabbit and goat ventricular myocardium sections (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359; Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Immunohistochemistry Analysis: A representative lot detected GRP94 immunoreactivity in fibrillating goat atria (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Western Blotting Analysis: Representative lots detected GRP94 in atrial myocytes from vairous species, including murine (Milano, G., et al. (2013). PLoS One. 8(10):e76659), goat and human (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206), and rabbit (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nNOS with GRP94 from chemically cross-linked C2C12 murine myoblasts in either proliferating or differentiating cultures (Vitadello, M., et al. (2014). Antioxid Redox Signal. 20(16):2479-2476).
Immunocytochemistry Analysis: A representative lot detected GRP94 immunoreactivity in murine C2C12 skeletal myoblasts as well as primary skeletal myocytes from new born mice (Gorza, L., and Vitadello, M. (2000). FASEB J. 14(3):461-475).
Immunofluorescence Analysis: Representative lots detected GRP94 immunoreactivity in rabbit and goat ventricular myocardium sections (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359; Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Immunohistochemistry Analysis: A representative lot detected GRP94 immunoreactivity in fibrillating goat atria (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
Western Blotting Analysis: Representative lots detected GRP94 in atrial myocytes from vairous species, including murine (Milano, G., et al. (2013). PLoS One. 8(10):e76659), goat and human (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206), and rabbit (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nNOS with GRP94 from chemically cross-linked C2C12 murine myoblasts in either proliferating or differentiating cultures (Vitadello, M., et al. (2014). Antioxid Redox Signal. 20(16):2479-2476).
Immunocytochemistry Analysis: A representative lot detected GRP94 immunoreactivity in murine C2C12 skeletal myoblasts as well as primary skeletal myocytes from new born mice (Gorza, L., and Vitadello, M. (2000). FASEB J. 14(3):461-475).
品質
Evaluated by Western Blotting in murine myoblast C2C12 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected GRP94 in 10 µg of murine myoblast C2C12 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected GRP94 in 10 µg of murine myoblast C2C12 cell lysate.
標靶描述
~94/110 kDa observed
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
其他說明
Concentration: Please refer to lot specific datasheet.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Sofia E Gomes et al.
PloS one, 13(1), e0191607-e0191607 (2018-01-24)
MicroRNAs (miRNAs) regulate a wide variety of biological processes, including tumourigenesis. Altered miRNA expression is associated with deregulation of signalling pathways, which in turn cause abnormal cell growth and de-differentiation, contributing to cancer. miR-143 and miR-145 are anti-tumourigenic and influence
Maurizio Vitadello et al.
Arthritis research & therapy, 12(2), R52-R52 (2010-03-26)
The endoplasmic reticulum (ER) stress-response, evoked in mice by the overexpression of class I major histocompatibility complex antigen (MHC-I), was proposed as a major mechanism responsible for skeletal muscle damage and dysfunction in autoimmune myositis. The present study was undertaken
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