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MABT523

Sigma-Aldrich

Anti-beta-Actin Antibody, clone RM112

clone RM112, from rabbit

同義詞:

Actin, cytoplasmic 1, Beta-actin, beta-Actin

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

RM112, monoclonal

物種活性

human

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... ACTB(60)

一般說明

Actin is a 43 kDa highly conserved structural protein. There are three main actin isotypes (alpha, beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

免疫原

A peptide corresponding to the N-terminus of beta-Actin.

應用

Immunocytochemistry Analysis (ICC): A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.

標靶描述

~45 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

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水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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