MABT388

Anti-ADAM 17 Antibody, clone 16C11.1 | MABT388

clone 16C11.1, from mouse

• 同義詞: Disintegrin and metalloproteinase domain-containing protein 17, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, CD156b
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 16C11.1, monoclonal
• 物種活性: human
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_003174
• UniProt登錄號: P78536
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ADAM17(6868)

描述

一般說明

Disintegrin and metalloproteinase domain-containing protein 17 (EC 3.4.24.86; UniProt P78536; also known as ADAM 17, CD156b, Snake venom-like protease. TNF-alpha convertase, TNF-alpha-converting enzyme, Tumor necrosis factor alpha-converting enzyme) is encoded by the ADAM17 (also known as CD156B, CSVP, NISBD, NISBD1, TACE) gene (Gene ID 6868) in human. Originally described as a TNF–alpha converting enzyme, ADAM17 is known to process the cleavage of many transmembrane proteins, including membrane-anchored cytokines, cell adhesion molecules, receptors, ligands, and enzymes. ADAM17 was the first ′sheddase′ identified. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. ADAM 17 is initially translated in its prepro-form and subsequent cleavages of the signal (aa 1-17) and propeptide (18-214) sequences produces the 610 aa mature ADAM 17 (aa 215-824).

特異性

Expected to react with both spliced isoforms.

免疫原

GST-tagged recombinant protein corresponding to human ADAM 17.

應用

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This mouse Monoclonal Anti-ADAM 17 Antibody, clone 16C11.1, Cat. No. MABT388 detects levels of ADAM17, and has been published and validated for use in Western Blotting, Immunohistochemistry (Paraffin).

Western Blotting Analysis: 5.0 µg/mL from a representative lot detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ADAM 17 in human testis and colorectal cancer tissues.

品質

Evaluated by Western Blotting in human acute myelogenous leukemia KG-1 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ADAM 17 in 10 µg of human acute myelogenous leukemia KG-1 cell lysate.

標靶描述

~120 kDa observed. A lower band ~100 kDa is also observed when using a higher antibody concentration (5 µg/mL). Due to glycosylation, target band size appears larger than the calculated molecular weights (93.0 kDa/prepro-isoform A, 68.2 kDa/mature isoform A, 78.5kDa/prepro-isoform B, 63.7 kDa/mature isoform B). Uncharacterized band(s) may appear in some lysates.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable