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Merck
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重要文件

MABS417

Sigma-Aldrich

Anti-GOT2, clone 3E9 Antibody

ascites fluid, clone 3E9, from mouse

同義詞:

Aspartate aminotransferase, mitochondrial, mAspAT, Fatty acid-binding protein, FABP-1, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine-oxoglutarate transaminase 4, Kynurenine--oxoglutarate

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
暫時無法取得訂價和供貨情況

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

3E9, monoclonal

物種活性

mouse, rat, monkey, human

技術

immunofluorescence: suitable
western blot: suitable

同型

IgG1

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... GOT2(2806)

一般說明

Aspartate aminotransferase, mitochondrial ((mAspAT), also known as Fatty acid-binding protein (FABP-1) or Glutamate oxaloacetate transaminase 2 or Kynurenine aminotransferase 4 or Kynurenine aminotransferase IV or Kynurenine--oxoglutarate transaminase 4 or Kynurenine--oxoglutarate transaminase IV or Plasma membrane-associated fatty acid-binding protein (FABPpm) or Transaminase A, and encoded by the gene name GOT2, plays an essential role in role in amino acid metabolism and facilitates cellular uptake of long-chain free fatty acids. GOT2 is localized to the mitochondrion; however, exposure to alcohol promotes translocation to the cell membrane.

免疫原

Purified recombinant fragment of human GOT2 expressed in E. Coli

應用

Anti-GOT2, clone 3E9 Antibody is an antibody against GOT2 for use in western blotting & immunofluorescence.
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GOT2 in PC-3 and SK-BR-3 cells.
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors

品質

Evaluated by Western Blotting in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GOT2 in HEK293, PC-12, BCBL-1, HepG2 and NIH/3T3 cell lysates.

標靶描述

~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外觀

Unpurified
Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Jing Xu et al.
Translational stroke research, 11(3), 418-432 (2019-09-02)
The preservation of mitochondrial function is a major protective strategy for cerebral ischemic injuries. Previously, our laboratory demonstrated that protein kinase C epsilon (PKCε) promotes the synthesis of mitochondrial nicotinamide adenine dinucleotide (NAD+). NAD+ along with its reducing equivalent, NADH

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