MABS278

Anti-EMAPII Antibody, clone M7/1, (Preservat ive free)

clone M7/1, from rat

• 同義詞: multisynthetase complex auxiliary component p43, multisynthetase complex auxiliary component 1, ARS-interacting multifunctional protein 1, Aminoacyl tRNA synthase complex-interacting multifunctional protein, Multisynthase complex auxiliary component p43,
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: M7/1, monoclonal
• 物種活性: human
• 物種活性（以同源性預測）: mouse (immunogen homology)
• 技術: activity assay: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_031952
• UniProt登錄號: P31230
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... AIMP1(9255)

描述

一般說明

Endothelial monocyte-activating polypeptide II (EMAPII) is a 22 kDa pro-inflammatory cytokine that regulates TNFβ signaling. The mature EMAPII is formed from a functional EMAPII precursor protein in response to apoptosis, and other forms of cell stress. Previous studies have reported that it may decrease the growth of tumors by inhibiting angiogenesis. EMAPII has also been implicated in autoimmune diseases. However, EMAPII is also involved in a range of normal biological processes. It is a component of the multisynthase complex that binds tRNA and plays an essential role in protein translation, and may also play a role in lung development.

特異性

This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

免疫原

His-tagged recombinant protein corresponding to mouse EMAPII.

應用

Anti-EMAPII Antibody, clone M7/1, (Preservat ive free) detects level of EMAPII & has been published & validated for use in Western Blotting, Activity Assay & IHC.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Western Blot Analysis: A representative lot was used by an independent laboratory in recombinant EMAP II proteins. (Rajashekhar, G., et al. (2009). J Immunol Methods. 350(1-2):22–28.)

Neutralization Antibody: A representative lot was used by an independent laboratory to functionally neutralize pro and mature forms of EMAPII in lung tissue lysate. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in lung parenchyma of patients with emphysema. (Clauss, M., et al. (2012). J Clin Invest doi:10.1172/JCI43881.)

品質

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected EMAPII in 10 µg of HeLa cell lysate.

標靶描述

~43 kDa observed. This antibody recognizes the mature form of this protein at ~23 kDa and the larger pro EMAP II form at ~43 kDa which is secreted based on lysate.

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing PBS without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HeLa cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable