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MABS1281

Sigma-Aldrich

Anti-Itk Antibody, clone 2F12

clone 2F12, from mouse

同義詞:

Tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
暫時無法取得訂價和供貨情況

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

2F12, monoclonal

物種活性

human

物種活性(以同源性預測)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

技術

immunoprecipitation (IP): suitable
western blot: suitable

同型

IgG1κ

NCBI登錄號

UniProt登錄號

運輸包裝

ambient

目標翻譯後修改

unmodified

基因資訊

human ... ITK(3702)

相關類別

一般說明

Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2; UniProt Q08881; also known as IL-2-inducible T-cell kinase, Interleukin-2-inducible T-cell kinase, Kinase EMT) (T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LPFS1, LYK) gene (Gene ID 3702) in human. Interleukin-2-inducible T-cell kinase (Itk) is a TEC family non-receptor tyrosine kinase that plays an important roles in signal transduction in T-cells. Upon cell surface receptor stimulation, Itk is recruited to the membrane by the adaptor molecule LAT (linker for activated T cells), where Itk is transphosphorylated at Tyr511 by Lck, Itk Tyr180 then takes place for full activation. Once activated, Itk transduces cellular signaling by activating phospholipase Cγ1 (PLCγ1), leading to activation of nuclear factor of activated T-cells (NFAT), nuclear factor κB (NFκB), and mitogen-activated protein kinase (MAPK) pathways. Itk-/- mice are resistant to developing ovalbumin-induced asthma and exhibit reduced cytokines production by iNKT cells, as well as reduced Th2 effector cytokines and IL-17A productin by T-cells.

特異性

Clone 2F12 targets the N-terminal region of human, mouse, and rat Itk.

免疫原

GST-tagged recombinant human/mouse/rat Itk N-terminal fragment.

應用

Detect ITK using this mouse monoclonal Anti-Itk Antibody, clone 2F12, Cat. No. MABS1281, validated for use in Immunoprecipitation and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A 1:1000 dilution from a representative lot detected Itk in 10 µg of HuT-78 human T-cell lymphoma cell lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Itk from both parental and Lck-negative (J.CaM1) Jurkat cells. Itk immunoprecipitated from the parental Jurkat cells was found with increased tyrosine phosphorylation following TCR cross-linking or pervanadate treatment, while only very weak tyrosine phosphorylation was seen with Itk from J.CaM1 upon prolonged pervanadate treatment (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).

Immunoprecipitation Analysis: A representative lot immunoprecipitated tyrosine-phosphorylated Itk from Jurkat cells stimulated by cross-linking of endogenous cell surface CD3 or transfected CD8 extracellular domain/CD28 cytoplasmic domain chimera (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).

Western Blotting Analysis: A representative lot detected baculovirus expressed Itk from infected Sf9 insect cells following ltk immunoprecipitation by the same antibody. The overexpressed ltk was found autophosphorylated on tyrosine when expressed alone in Sf9 and increased ltk tyrosine phosphorylation was seen when co-expressed with Lck (Heyeck, S.D., et al. (1997). J. Biol. Chem. 272(40):25401-25408).

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Itk in 10 µg of Jurkat cell lysate.

標靶描述

~71 kDa observed. 71.83/72.29/72.49 (human/mouse/rat) kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified.
Format: Purified
Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Tone Berge et al.
PloS one, 5(3), e9761-e9761 (2010-03-23)
The chemokine CXCL12/SDF-1alpha interacts with its G-protein coupled receptor CXCR4 to induce migration of lymphoid and endothelial cells. T cell specific adapter protein (TSAd) has been found to promote migration of Jurkat T cells through interaction with the G protein

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