MABN14

Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3

clone 1D3, from rat

• 同義詞: TAR DNA-binding protein 43, TDP-43, TDP43, TAR DNA binding protein-43, Phosphorylated TDP
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1D3, monoclonal
• 物種活性: human
• 技術: ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_031401
• UniProt登錄號: Q13148
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer409/pSer410)
• 基因資訊: human ... TARDBP(23435)

描述

一般說明

TAR DNA-binding protein 43 (TDP-43) belongs to the hnRNP protein family and plays an important role in transcription, pre-mRNA splicing, mRNA stability and mRNA transport. It is involved in splicing of the apolipoprotein A-II and cystic fibrosis transmembrane gene. This protein is highly expressed in the pancreas, placenta, lung, genital tract and spleen. Mutations in TDP-43 have been associated with amyotrophic lateral sclerosis, frontotemporal dementia, Parkinson′s disease and Alzheimer′s disease.

特異性

This antibody recognizes TDP-43 when phosphorylated at Ser409/Ser410.

免疫原

Epitope: Ser409/Ser410

Ovalbumin-conjugated linear peptide corresponding to human TDP-43 phosphorylated at Ser409/Ser410.

應用

Anti-phospho TDP-43 (Ser409/Ser410) Antibody, clone 1D3 is an antibody against phospho TDP-43 (Ser409/Ser410) for use in WB, ELISA, IH.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in hippocampus from FTLD-U brain tissue. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)

ELISA Analysis: A previous lot was used by an independent laboratory in ELISA. (Neumann, M., et al. (2009). Acta Neuropathol. 117:137–149.)

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

品質

Evaluated by Western Blot in human placenta cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected phosphorylated TDP-43 on 10 µg of human placenta cell lysate.

標靶描述

50/25 kDa Observed
1) The 25-kDa C-terminal fragment is more prone to phosphorylation at Ser409/Ser410 than full-length TDP-43. (Zhang, Y.J., et al. (2009). PNAS. 106:7607-7612.)
2) 50-52 kDa observed MW . (Buratti, E., et al. (2001). The EMBO Journal 20:1774-1784.)

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Human placenta cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10-13 - German Storage Class 10 to 13