MABF2115

Anti-NKR-P1B (CD161b) Antibody, clone 2D12

• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 抗體表格: purified antibody
• 無性繁殖: 2D12; monoclonal
• 純化經由: using protein G
• 物種活性: mouse, mouse, mouse
• 濃度: (Please refer to lot specific datasheet.)
• 技術: flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2aλ
• 適合性: suitable for immunoassay
• UniProt登錄號: Q99JB4

描述

一般說明

Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.

特異性

Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.

應用

Affects Function Analysis: A representative lot reversed the reduction in killing induced by the expression of Clrb in C1498, BW5147, and B6 LAK cells. (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1B (CD161b) in Immunoprecipitation applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7; Aguilar, O.A., et. al. (2017). Cell. 169(1):58-71).

品質

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKR-P1B (CD161b) in mouse spleen tissue lysate.

物理性質

~52 kDa observed; 25.03 kDa calculated. . Uncharacterized bands may be observed in some lysate(s).

外觀

Purified mouse monoclonal antibody IgG2a in PBS without azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.