MABF1968

Anti-Microglobulin beta2 Antibody, clone BBM.1

clone BBM.1, from mouse

• 同義詞: B2M
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: BBM.1, monoclonal
• 物種活性: human
• 包裝: antibody small pack of 25 μg
• 技術: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); inhibition assay: suitable; western blot: suitable
• 同型: IgG2bκ
• NCBI登錄號: NP_004039
• UniProt登錄號: P61769
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... B2M(567)

描述

一般說明

Beta-2-microglobulin (UniProt: P61769) is encoded by the B2M (also known as CDABP0092, HDCMA22P) gene (Gene ID: 567) in human. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC) that is involved in the presentation of peptide antigens to the immune system. It is synthesized with a signal peptide of 20 amino acids, which is subsequently cleaved to generate the mature form. It also contains an Ig-like C1 type domain (aa 25-113). Beta-2-microglobulin lacks a transmembrane anchoring domain and is found in secreted as well as in complex. Serum beta-2-microglobulin levels are a reflection of cell turnover and its levels are shown to be elevated in fever, inflammation, and infection. Increased serum levels are also seen in B-cell malignancies and in renal failure and may indicate a worse prognosis for patients with early-stage Hodgkin′s lymphoma. Its levels may be elevated either due to higher rates of synthesis as observed in HIV infection and malignancy or due to impaired renal filtration due to renal insufficiency.

特異性

Clone BBM.1 detects Beta-2-microglobulin in human.

免疫原

MOLT-4, a Human acute lymphoblastic leukemia cell line.

應用

Anti-Microglobulin beta2, clone BBM.1, Cat. No. MABF1968, is a mouse monoclonal antibody that detects Microglobulin beta2 and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition assays, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected Microglobulin beta2 in Immunohistochemistry applications (Josson, S., et. al. (2011). Cancer Res. 71(7):2600-10).

Inhibition Analysis: A representative lot inhibited Microglobulin beta2 activity (Parham, P., et. al. (1983). J Biol Chem. 258(10):6179-86).

Flow Cytometry Analysis: A representative lot detected Microglobulin beta2 in Flow Cytometry applications (Drukker, M., et. al. (2012). Nat Biotechnol. 30(6):531-42).

Immunohistochemistry Analysis: A 1:50-1:1,000 dilution from a representative lot detected Microglobulin beta2 in human breast cancer and human tonsil tissues.

Research Category
Inflammation & Immunology

品質

Evaluated by Western Blotting in MOLT-4 cell lysates

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Microglobulin beta2 in 10 µg of MOLT-4 cell lysate.

標靶描述

~14 kDa observed; 13.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1