MABE918

Anti-Brd4 Antibody, clone 1F11

clone 1F11, from mouse

• 同義詞: Bromodomain-containing protein 4, Protein HUNK1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1F11, monoclonal
• 物種活性: human
• 技術: ChIP: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_055114
• UniProt登錄號: O60885
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... BRD4(23476)

描述

一般說明

Bromodomain-containing protein 4 (Brd4), or Mitotic chromosome-associated protein (MCAP), is a chromatin reader protein and kinase that recognizes and binds acetylated histones and plays a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Brd4 remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, Brd4 plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Brd4 is widely expressed and localized to the nucleus and acetylated chromatin. Brd4 also plays a large role in many cancers, and through its regulation of transcription, Brd4 is important in multiple cellular processes including DNA damage responses, aging, and development.

免疫原

Epitope: a.a. 785-1055 domain

Recombinant protein corresponding to the a.a. 785-1055 domain of human Brd4.

應用

Anti-Brd4, clone 1F11 is an antibody against Brd4 for use in WB, ChIP.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Brd4 using breast cancer cells MCF-7 (Data courtesy of Cheng-Ming Chiang, Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas).

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Brd4 in 16 µg of HeLa cell lysate.

標靶描述

~152 kDa observed. Uncharacterized band(s) may appear in some lysates.

外觀

Format: Purified

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2