MABE1060

Anti-CtIP Antibody, clone 14-1

clone 14-1, from mouse

• 同義詞: CtBP-interacting protein, CtIP, Retinoblastoma-binding protein 8, RBBP-8, Retinoblastoma-interacting protein and myosin-like, RIM, Sporulation in the absence of SPO11 protein 2 homolog, SAE2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 14-1, monoclonal
• 物種活性: mouse, human
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_002885.1
• UniProt登錄號: Q99708
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RBBP8(5932)

描述

一般說明

DNA endonuclease RBBP8 (UniProt: Q99708; also known CtBP-interacting protein, CtIP, Retinoblastoma-binding protein 8, RBBP-8, Retinoblastoma-interacting protein and myosin-like, RIM, Sporulation in the absence of SPO11 protein 2 homolog, SAE2) is encoded by the RBBP8 (also known as CTIP) gene (Gene ID: 5932) in human. RBBP-8 is an endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. RBBP8 is phosphorylated upon DNA damage (at Thr859) by ATR and this phosphorylated form binds to chromatin to initiate resection. The non-phosphorylated form does not bind to chromatin. It is reported that ATM activity is required for an early step in resection, leading to ATR activation, CtIP- phosphorylation, and accumulation of CtIP on chromatin. RBBP8 functions downstream of the MRN complex and promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. It is localized predominantly in the nucleus and interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. By interacting with BCRA1 it modulates transcriptional regulation, DNA repair, and cell cycle checkpoint control. Mutations in RBBP8 gene are known to cause Seckel syndrome 2, an autosomal recessive disorder characterized by proportionate dwarfism of prenatal onset associated with low birth weight, growth retardation, severe microcephaly, and mental retardation.

特異性

Clone 14-1 specifically detects CtBP-interacting protein in HEK293 cells.

免疫原

Epitope: domain of:

GST-tagged recombinant fragment corresponding to 278 amino acids from the C-terminal half of human CtBP-interacting protein.

應用

Anti-CtIP Antibody, clone 14-1, Cat. No. MABE1060, is a highly specific mouse monoclonal antibody that targets CtIP and has been used in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected CtIP in Immunocytochemistry applications (Murina, O., et. al. (2014). Cell Rep. 7(4):1030-8).

Western Blotting Analysis: A representative lot detected CtIP in Western Blotting applications (Yu, X., et. al. (2000). J Biol Chem. 275(24):18541-9; Bunting, S.F., et. al. (2010). Cell. 141(2):243-54; Wu, M., et. al. (2007). Mol Cancer Res. 5(12):1285-95).

Immunoprecipitation Analysis: A representative lot was used for CtIP Immunoprecipitation. (Yu, X., et. al. (2000). J Biol Chem. 275(24):18541-49).

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in HEK293 cell lysate.


Western Blotting Analysis: 0.5 µg/mL of this antibody detected CtIP in 10 µg of HEK293 cell lysate.

標靶描述

~120 kDa observed; 101.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1