MABC954

Anti-MUTYH Antibody, clone 12D5.1

clone 12D5.1, from mouse

• 同義詞: A/G-specific adenine DNA glycosylase, MutY homolog, hMYH
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 12D5.1, monoclonal
• 物種活性: human
• 技術: western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_036354
• UniProt登錄號: Q9UIF7
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MUTYH(4595)

描述

一般說明

A/G-specific adenine DNA glycosylase (UniProt Q9UIF7; also known as hMYH, MutY homolog) is encoded by the MUTYH (also known as MYH) gene (Gene ID 4595) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. MUTYH/MYH is a monofunctional DNA glycosylase that excises mismatched adenine opposite 7, 8-dihydro-8-oxoguanine (8-oxoG), which is the major mutagenic lesion induced by oxidative stress. Biallelic mutations in MUTYH are associated with MUTYH-Associated polyposis (MAP) and increased risk in colorectal cancer (CRC).

特異性

Expected to react with all six spliced isoforms of human MUTYH reported by UniProt (Q9UIF7).

免疫原

Epitope: N-terminal half.

GST-tagged recombinant internal fragment from the N-terminal half of human MUTYH.

應用

Anti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Cell Cycle, DNA Replication & Repair

品質

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MUTYH in 10 µg of Raji cell lysate.

標靶描述

~59 kDa observed. 60.07 kDa (isoform Alpha-1), 59.14 kDa (isoform Alpha-2), 59.07 kDa (isoform Alpha-3), 58.44 kDa (isoform Beta-1), 57.52 kDa (isoform Gamma-2), and 50.52 kDa (isoform Gamma-3) calculated.

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable