MABC593

Anti-Astrin/SPAG5, clone 8B2 Antibody

clone 8B2, from rat

• 同義詞: Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 8B2, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 同型: IgG2cκ
• NCBI登錄號: NP_006452
• UniProt登錄號: Q96R06
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SPAG5(10615)

描述

一般說明

Astrin/SPAG5 is a microtubule binding and cytoskeleton associated signaling protein that plays not only a critical role in mitotic spindle and chromosome segregation but also as an important signal transducer in mTOR and GSK3 beta signaling pathways. Astrin/SPAG5 is localized in the cytoplasm and/or mitotic apparatus (in dividing cells) and centrosomes. In non-dividing cells Astrin/SPAG5 is associated with microtubules unless under cellular stress, then it is localized in stress granules. Astrin/SPAG5 is expressed in many tissues but is very high in testis and sperm and is regulated via the cell cycle highest levels are seen in prophase. Recent research has focused on Astrin/SPAG5 critical role in regulating mTORC1 and its effects on growth and survival. When cells are stress Astrin/SPAG5 inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules (SGs), thereby preventing mTORC1-hyperactivation-induced apoptosis. Because cancerous tumor cells are often stressed, this mechanism is believed to be key to their survival, thus Astrin/SPAG5 activity repression is being examined as potential anti-cancer therapy.

免疫原

Linear peptide corresponding to human Astrin/SPAG5.

應用

Immunocytochemistry Analysis: A representative lot detected Astrin/SPAG5 HeLa cells (Thedieck, K., et al. (2013). Cell. 154:859-874).
Western Blotting Analysis: A representative lot detected Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Astrin/SPAG5, clone 8B2 Antibody is validated for use in Immunocytochemistry and Western Blotting and Immunoprecipitation for the detection of Astrin/SPAG5.

品質

Evaluated by Immunocytochemistry in HeLa and A431 cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Astrin/SPAG5 in HeLa and A431 cells.

標靶描述

134 kDa calculated

外觀

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable