推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
DB7, monoclonal
物種活性
human, rabbit, guinea pig
應無反應活性
rat, mouse
製造商／商標名
Chemicon®
技術
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同型
IgG2a
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... TNC(3371)
特異性
The antibody recognizes the Mr 250000 and 180000 tenascin polypeptides in immunoprecipitation and immunoblots the Mr 250000 polypeptide. The antibody reacts with the fibrinogen-like knob-domain of tenascin protein
免疫原
Tenascin polypeptides isolated from spent culture supernatant of human fibroblasts isolated by affinity chromatography.
應用
Detect Tenascin using this Anti-Tenascin Antibody, clone DB7 validated for use in IF, WB, IH(P).
Immunofluorescence: 1-2% PFA fixed, permeabilized tissues or cell cultures. Mild pepsin digestion improves staining.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Immunohistochemistry (formalin-fixed and paraffin-embedded sections): 1:200-1:400.
Requires pepsin digestion (0.1-.4% pepsin in .1% HCL pH 1.0, 30-50 minutes at 37C; neutralize sections with 2 quick rinses in 1X PBS pH 7.4 prior to blocking and antibody staining. Enhanced ABC or amplified detection systems recommended for best signals. See Soini, Y 1993 J Clin Patholo for protocol for decalified bone.
Immunoblotting: 1:1000; SDS sample buffer lysis of whole human fibroblasts; 6.5% PAGE reduced gels. Two distinct bands, 250,000 & 190,000 {Soini, Y Am. J. Clin. Path. 1993)
Final dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
聯結
Replaces: MAB1902
外觀
Format: Purified
The purified antibody is in PBS solution containing 1.0% (w/v) BSA and 0.1% (w/v) sodium azide.
儲存和穩定性
Maintain refrigerated at 2-8°C
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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