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Key Documents

MAB1330

Sigma-Aldrich

Anti-Collagen Type II Antibody, clone COLL-II

ascites fluid, clone COLL-II, Chemicon®

同義詞:

Anti-ANFH, Anti-AOM, Anti-COL11A3, Anti-SEDC, Anti-STL1

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

ascites fluid

抗體產品種類

primary antibodies

無性繁殖

COLL-II, monoclonal

物種活性

sheep, human, bovine, rat, pig, mouse, canine

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry: suitable

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... COL2A1(1280)

特異性

Reacts with both pepsin-solubilized and CNBr-cleaved human and bovine collagen type II. No cross-reactivity with collagen types I, III, V and IX.

免疫原

Human (cartilage specific) CNBr-cleaved collagen II.

應用

Immunofluorescence: 1:2-1:5 with fresh, frozen or acetone-fixed material. Mild pepsin digestion is recommended to enhance staining.

Western Blot: 1:1000-1:2000 with chemiluminescent detection

ELISA at 1:100-1:500, pepsin treated samples.





Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-Collagen Type II Antibody, clone COLL-II is validated for use in ELISA, IH for the detection of Collagen Type II.

外觀

Unpurified
Ascites fluid containing no preservatives.

儲存和穩定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析報告

Control
Cartilage in lung or fetus

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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Frederic Cailotto et al.
The Journal of biological chemistry, 285(14), 10572-10582 (2010-02-06)
The differentiated phenotype of chondrocyte is lost in pathological situations and after interleukin (IL)-1beta challenge. Wnt proteins and the inorganic pyrophosphate (PP(i)) transporter Ank regulate the differentiation process in many cell types. We investigated the possible contribution of Ank and/or
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Orthopaedic surgery, 10(1), 56-63 (2018-02-13)
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Journal of cellular and molecular medicine, 16(4), 877-887 (2011-07-19)
Irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (MSC), which play a pivotal role in bone regeneration. As a consequence of radiotherapy, bones may develop osteoradionecrosis. When irradiating human bone-derived MSC in vitro with increasing
L A Vonk et al.
Osteoarthritis and cartilage, 22(1), 145-153 (2013-11-26)
Hsa-miR-148a expression is decreased in Osteoarthritis (OA) cartilage, but its functional role in cartilage has never been studied. Therefore, our aim was to investigate the effects of overexpressing hsa-miR-148a on cartilage metabolism of OA chondrocytes. OA chondrocytes were transfected with

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