ZRB1201

Anti-Collagen Type II Antibody, clone 1K11 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Collagen alpha-1(II) chain;Alpha-1 type II collagen
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1K11, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 141.79 kDa; observed mol wt ~140 kDa
• 純化經由: using Protein A
• 物種活性: human, chicken
• 物種活性（以同源性預測）: equine, porcine, monkey, bovine, feline, canine
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal half
• Protein ID登錄號: NP_001835.3
• UniProt登錄號: P02458
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... COL2A1(1280)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

免疫原

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Collagen type II.

應用

Quality Control Testing

Evaluated by Western Blotting on Pepsin digest of Chicken sternal collagen Type II.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Pepsin digest of Chicken sternal collagen Type II.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Collagen Type II in Human chondrocytes.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Collagen Type II in Human cartilage tissue sections.

Flow Cytometry Analysis: 1 μg from a representative lot detected Collagen Type II in one million Human chondrocytes.

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected purified Human Collagen Type II.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting on Pepsin digest of Chicken sternal collagen Type II.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Pepsin digest of Chicken sternal collagen Type II.

標靶描述

Collagen alpha-1(II) chain (UniProt: P02458; also known as Alpha-1 type II collagen) is encoded by the COL2A1 gene (Gene ID: 1280) in human. Collagen is the most abundant protein in the body that is predominantly synthesized by fibroblasts. However, epithelial cells may also synthesize small amounts of collagen. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Collagens contain a Gly-X-Y repeating structure. The glycine residue is necessary in every third position to ensure close packaging. Although any amino acid can be present at position X or Y, proline is more frequently found in the X position and hydroxyproline in the Y position. Both proline and hydroxyproline stabilize the helical conformation of polypeptide chains. This triplet of amino acids allows collagen chains to twist into a helical structure ( -helix) forming a super helix. Each collagen molecule contains 3 chains twisted around each other to form a triple helix. These 3 chains may either be identical or different depending on the type of collagen. Type II collagen is a major constituent of cartilaginous tissues, representing 90 95% of its total collagen content and forming the fibrils that give cartilage its tensile strength. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. It is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 26-181), which are subsequently cleaved off to produce mature collagen. Cleavage at 181-182 residues is brought about by N-endopeptidases. Excessive cleavage of type II collagen by collagenases is observed in cases of osteoarthritis (OA). This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Cahue, S., et al.(2007). Osteoarthritis Cartilage. 15(7); 819-823; Poole, AR., et al. (2002). Ann. Rheum. Dis. 61(Suppl II); 78-81).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1