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MAB10764

Sigma-Aldrich

抗SBP标签抗体,克隆20|MAB10764

clone 20, 1.0 mg/mL, from mouse

同義詞:

streptavidin binding peptide

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

20, monoclonal

物種活性

human

濃度

1.0 mg/mL

技術

immunocytochemistry: suitable
western blot: suitable

同型

IgG1κ

運輸包裝

wet ice

目標翻譯後修改

unmodified

一般說明

链霉亲和素结合肽标签是一种有用且用途广泛的肽,可以插入表达载体中,以便于重组蛋白的检测和纯化。SBP标签的序列长38个氨基酸,对链霉亲和素具有纳摩尔规模结合亲和力,平衡常数为2.5 nM。

特異性

证明与表达SBP标签的细胞反应。
该抗体识别SBP标签。

免疫原

对应于SBP标签的线性肽。
表位:未知

應用

免疫荧光分析: 先前批次已被独立实验室用于IF。(Hudson, D., et al. (2008).MBC. 19(7):3070-3079.)
研究子类别
表位标签
研究类别
表位标签 & 一般用途

品質

已通过蛋白质印迹在pTrAP-GFP转染的Hek293裂解物中进行了评估。

蛋白质印迹分析:0.1 ng/ml的该抗体在10 µg pTrAP-GFP转染的Hek293裂解物上检测到SBP标签。

標靶描述

蛋白表达是包含组氨酸标签,S标签,链霉亲和素结合肽(SBP)标签和GFP的pTrAP-GFP质粒。表达的蛋白约为37kDa

外觀

在含 0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl) 和 0.05% 叠氮化钠的缓冲液中的纯化的小鼠单克隆 IgG1κ。
形式:纯化
纯化的蛋白G

儲存和穩定性

自收到之日起,在 2-8°C 条件下可稳定保存1年

分析報告

对照
pTrAP-GFP转染的Hek293裂解物

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

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Methods in molecular biology (Clifton, N.J.), 1174, 211-223 (2014-06-21)
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