The normal sample ranges provided are intended as a general guideline and should not be considered a true reference range due to being based on a small sample size and lacking randomization.
HMYOMAG-56K
MILLIPLEX® 人肌细胞因子磁珠板
The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.
About This Item
推薦產品
品質等級
物種活性
human
製造商/商標名
Milliplex®
assay range
accuracy: 100%
(Apelin)
accuracy: 92%
(IL-15)
accuracy: 96%
(FSTL-1)
accuracy: 97%
(LIF)
accuracy: 98%
(Oncostatin M (OSM))
sensitivity: 0.9 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 14 pg/mL
(MinDC+2SD; FGF-21)
sensitivity: 259 pg/mL
(MinDC+2SD; Myostatin/GDF8)
sensitivity: 281 pg/mL
(MinDC+2SD; Irisin)
sensitivity: 4 pg/mL
(MinDC+2SD; LIF)
sensitivity: 40 pg/mL
(MinDC+2SD; Fractalkine/CX3CL1)
sensitivity: 5 pg/mL
(MinDC+2SD; IL-15)
sensitivity: 59 pg/mL
(MinDC+2SD; Osteocrin/Musclin)
sensitivity: 6 pg/mL
(MinDC+2SD; Oncostatin M (OSM))
sensitivity: 64 pg/mL
(MinDC+2SD; Apelin)
sensitivity: 7.5 ng/mL
(MinDC+2SD; Osteonectin (SPARC))
sensitivity: 737 pg/mL
(MinDC+2SD; Follistatin-like Protein 1 (FSTL1))
sensitivity: 815 pg/mL
(MinDC+2SD; Erythropoietin (EPO))
sensitivity: 9 pg/mL
(MinDC+2SD; BDNF)
sensitivity: 9.8 pg/mL
(MinDC+2SD; FABP3)
standard curve range: 0.6-2,400 pg/mL
(IL-6)
standard curve range: 10-40,000 pg/mL
(LIF)
standard curve range: 122-500,000 pg/mL
(Apelin)
standard curve range: 2-10,000 pg/mL
(BDNF)
standard curve range: 2-10,000 pg/mL
(IL-15)
standard curve range: 2-6,000 pg/mL
(Oncostatin M (OSM))
standard curve range: 24-100,000 pg/mL
(FABP3)
standard curve range: 244-1,000,000 pg/mL
(Erythropoietin (EPO))
standard curve range: 244-1,000,000 pg/mL
(Irisin)
standard curve range: 488-2,000,000 pg/mL
(Follistatin-like Protein 1 (FSTL1))
standard curve range: 488-2,000,000 pg/mL
(Myostatin/GDF8)
standard curve range: 49-200,000 pg/mL
(Fractalkine/CX3CL1)
standard curve range: 49-200,000 pg/mL
(Osteocrin/Musclin)
standard curve range: 5-20,000 pg/mL
(FGF-21)
standard curve range: 7-30,000 ng/mL
(Osteonectin (SPARC))
inter-assay cv: <15%
intra-assay cv: <10%
(Apelin)
inter-assay cv: <15%
intra-assay cv: <10%
(BDNF)
inter-assay cv: <15%
intra-assay cv: <10%
(Erythropoietin (EPO))
inter-assay cv: <15%
intra-assay cv: <10%
(FABP3)
inter-assay cv: <15%
intra-assay cv: <10%
(FGF-21)
inter-assay cv: <15%
intra-assay cv: <10%
(Follistatin-like Protein 1 (FSTL1))
inter-assay cv: <15%
intra-assay cv: <10%
(Fractalkine/CX3CL1)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-15)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <15%
intra-assay cv: <10%
(Irisin)
inter-assay cv: <15%
intra-assay cv: <10%
(LIF)
inter-assay cv: <15%
intra-assay cv: <10%
(Myostatin/GDF8)
inter-assay cv: <15%
intra-assay cv: <10%
(Oncostatin M (OSM))
inter-assay cv: <15%
intra-assay cv: <10%
(Osteocrin/Musclin)
inter-assay cv: <20%
intra-assay cv: <10%
(Osteonectin (SPARC))
技術
multiplexing: suitable
檢測方法
fluorometric (Luminex xMAP)
運輸包裝
wet ice
一般說明
MILLIPLEX®人肌动蛋白面板是一种15重试剂盒,用于同时定量人血清、血浆和细胞/组织培养上清液样品中以下任何或所有分析物:Fractalkine/CX3CL1,BDNF,制瘤素M(OSM),骨连接蛋白(SPARC),LIF,FGF-21,促红细胞生成素(EPO),IL-15,IL-6,Apelin,Myostatin/GDF8,鸢尾素,Osteocrin/Musclin,FABP3和卵泡抑素样蛋白(FSTL 1)。该试剂盒采用96孔板,包含冻干标准混合物、两个内部检测质控品,可最多可测定38份样本,重复两次。
Luminex® xMAP®平台使用磁珠免疫分析格式,以实现理想的速度和灵敏度,同时定量多种分析物,从而显著提高生产力,同时节省宝贵的样本量。
面板类型:代谢
應用
- 分析物:Apelin(APLN)、脑源性神经营养因子(BDNF)、促红细胞生成素(EPO)、脂肪酸结合蛋白3(FABP3)、成纤维细胞生长因子21(FGF21)、Fractalkine(CX3CL1)、卵泡抑素样1蛋白(FSTL-1)、IL-6、IL-15、鸢尾素、白血病抑制性因子(LIF)、肌肉生长抑制素(MSTN/GDF8)、制瘤素M(OSM)、骨调节素(OSTN/Musclin)、骨连接蛋白(SPARC)
- 推荐的样品类型:人血清、血浆和细胞/组织培养上清液或裂解液
- 注释:大量的BDNF储存在循环的血小板中,随后在血小板活化后释放。因此,贫血小板血浆对于确保BDNF循环水平的准确测量至关重要。
- 推荐的样品稀释度:每孔25 μL 1:2稀释的血清或血浆。细胞/组织培养样品可以在未稀释或适当稀释的对照培养基中运行。
- 分析运行时间:在2-8℃下过夜(16-18小时)或在室温(20-25℃)下2小时。
- 研究类别:骨
- 研究子类别:代谢
特點和優勢
其他說明
法律資訊
訊號詞
Danger
危險分類
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2
標靶器官
Respiratory Tract
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
相關內容
Myokine analysis with multiplex immunoassays, such as the MILLIPLEX® Human Myokine Panel, allow researchers to simultaneously measure multiple muscle-secreted factors, providing more insights into related diseases.
多重免疫分析如 MILLIPLEX® 人類肌動蛋白面板可進行全面的肌動蛋白分析,提供對相關疾病的深入瞭解。
Simplify protein biomarker discovery with the MILLIPLEX® PLEXpedition 115-Plex Multiplex Screening Panel. Effortlessly screen cytokines and other markers with Luminex® technology.
使用 MILLIPLEX® PLEXpedition 115-Plex 多重篩選板簡化蛋白質生物標記的發現。利用 Luminex® 技術輕鬆篩檢細胞因子和其他標記物。
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Could you please provide the normal human serum/plasma sample ranges for the Human Myokine Milliplex kit?
1 answer-
Helpful?
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How does the sensitivity of 815 pg/mL convert to mIU/mL?
1 answer-
We have not tested and determined a conversion factor for this assay. The international unit (IU) for EPO has been defined by WHO based on in vivo activity in model animals. Currently, recombinant human EPO produced by CHO cells (1650 IU in approximately 11 μg of epoetin-α) is distributed in ampoules as the third WHO International Standard for the calibration of recombinant DNA-derived EPO activity by bioassay. Our standard of EPO is a recombinant human EPO.
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