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ECM535

Sigma-Aldrich

α/β Integrin-mediated Cell Adhesion Array Combo Kit, fluorimetric

The Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface.

同義詞:

Alpha/beta integrin cell adhesion kit

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32161000
NACRES:
NA.32

品質等級

物種活性

human

製造商/商標名

Chemicon®

技術

cell based assay: suitable

NCBI登錄號

檢測方法

fluorometric

運輸包裝

wet ice

基因資訊

human ... ITGA1(3672)

應用

Research Category
Cell Structure
The Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface.
The CHEMICON® Alpha/Beta Integrin-Mediated Cell Adhesion Array Kit can be used for assessing the presence or absence of specific integrins on the cell surface. This assay can substitute for FACS analysis (6) and is useful for screening human adherent cells for cell surface integrins or for monitoring in vitro cell differentiation or genetic modification of cells.

For Research Use Only. Not for use in diagnostic procedures.

包裝

96 wells each

成分

Alpha Integrin Array Plate: (PN: 90599) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-alpha integrin monoclonal antibodies: (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, alphavbeta3), and one goat anti-mouse negative well. See the Alpha Integrin plate layout on the insert data sheet.

Beta Integrin Array Plate: (PN: 90600) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-beta integrin monoclonal antibodies: (beta1, beta2, beta3, beta4, beta6, alphaVbeta5, and alpha5beta1), and one goat anti-mouse negative well. See the Beta Integrin plate layout on the insert data sheet.

4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL

CyQuant GR Dye: (Part No. 90132) Two vials - 75 μL each

Assay Buffer: (Part No. 90601) Two bottles - 100 mL each

儲存和穩定性

The Alpha and Beta Integrin Array Plates can be stored at 2° to 8°C in the foil pouch up to their expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.

Precautions

No data is available on the biological toxicity of CyQuant GR dye. This reagent binds to nucleic acids, and as such, should be treated as a potential mutagen which may cause cancer and heritable genetic damage. Handle with caution. The DMSO stock solution should be handled with special caution as DMSO can facilitate the entry of organic molecules into tissues.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
GR is a trademark of Sigma-Aldrich Co. LLC

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

CorrosionEnvironment

訊號詞

Danger

危險聲明

危險分類

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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P G Dedes et al.
Biochimica et biophysica acta, 1820(12), 1926-1939 (2012-08-14)
The extracellular matrix (ECM) components play key roles in the multistep process of cancer growth and progression. Preclinical and clinical data show that bisphosphonates (BPs) may exert direct or indirect antitumoral effects. Despite proven efficiency in cancer treatment, the mechanism

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