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DP02

Sigma-Aldrich

抗SV40 T抗原(Ab-2)小鼠单克隆抗体(PAb416)

liquid, clone PAb416, Calbiochem®

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

PAb416, monoclonal

形狀

liquid

包含

≤0.1% sodium azide as preservative

物種活性

SV40-infected cells

製造商/商標名

Calbiochem®

儲存條件

do not freeze

同型

IgG2a

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

一般說明

SV40 T Ag(Ab-2)是一种小鼠单克隆抗体,对SV40大T抗原特有的抗原决定簇具有特异性,与SV40小T抗原无反应性。两种抗原均由SV40基因组的早期区域编码。大的T抗原结合DNA,并与53,000道尔顿的细胞转化蛋白p53形成复合物,p53是裂解生长过程中启动病毒DNA复制所必需的。此外,大T抗原与DNA聚合酶和转录因子AP-2结合,并与视网膜母细胞瘤易感基因的p105产物形成特异性复合物。
识别SV80和SV-T2细胞中的~94 kDa SV40大T抗原。
该抗SV40 T抗原(Ab-2)小鼠mAb(PAb416)经验证可用于亲和纯化、免疫印迹、IF、IP和石蜡切片,以检测SV40 T抗原(Ab-2)。
通过用指定的免疫原免疫小鼠并将脾细胞与NS-1小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体(请参阅应用参考)。识别~94 kDa SV40大T抗原。

免疫原

纯化SV40大T抗原
表位:氨基酸83-128内

應用

亲和纯化(使用目录编号DP02A)

免疫印迹(1-5 µg/ml,请参阅应用参考)

免疫荧光(1-5 µg/ml)

免疫沉淀(1-5 µg抗体,使用目录编号DP02A)

石蜡切片(请参阅应用参考)

包裝

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外觀

溶于50 mM磷酸钠缓冲液(pH 7.5),含0.2%明胶。

分析報告

阳性对照
SV80或SV-T2细胞
阴性对照
3T3细胞

其他說明

DeCaprio, J.A., et al. 1988.Cell54, 275.
Whyte, P., et al. 1988.Nature334, 124.
Mitchell, P.J., et al. 1987.Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985.J. Virol.53, 1001.
Simanis, V. and Lane, D.P.1985.Virol.144, 88.
Mann, R.S. and Carroll, R.B.1984.Virology138, 379.
Sarnow, P., et al. 1982.Cell28, 387.
Crawford, L.V., et al. 1981.Proc.Natl.Acad.Sci. USA78, 41.
Lane, D.P. and Crawford, L.V.1979.Nature278, 261.
Carroll, R.B., et al. 1974.Proc.Natl.Acad.Sci. USA71, 3754.
Tooze, J. 1973.Cold Spring Harbor, New York.
Tegtmeyer, P. 1972.J. Virol.10, 591.
也可用琼脂糖偶联物(目录编号DP02A),适用于亲和纯化和免疫沉淀。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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