ABS2231

Anti-phospho RyR2 (Ser2808)

• 同義詞: RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 分子量: calculated mol wt 564.57 kDa; observed mol wt ~565 kDa
• 純化經由: affinity chromatography
• 物種活性: rabbit
• 物種活性（以同源性預測）: mouse, human
• 包裝: antibody small pack of 100 μg
• 技術: western blot: suitable
• 同型: IgG
• 表位序列: C-terminal cytoplasmic domain
• Protein ID登錄號: NP_001026
• UniProt登錄號: Q92736
• 儲存溫度: -10 to -25°C
• 目標翻譯後修改: phosphorylation (pSer2808)
• 基因資訊: human ... RYR2(55193)

描述

一般說明

Ryanodine receptor 2 (UniProt: Q92736; also known as RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor) is encoded by the RYR2 gene (Gene ID: 6262) in human. Ryanodine receptors are large ion channels that are located in the sarcoplasmic/endoplasmic reticulum membrane and are responsible for the release of Ca2+. They form homotetramers with a mushroom-like shape, consisting of a large cytoplasmic head and transmembrane stalk. In mammals three isoforms of RYR have been described (RYR 1-3) that are regulated by phosphorylation, redox modifications, and a variety of small proteins, and ions. Ryanodine receptor 2 is a multi-pass membrane protein with two cytoplasmic domains (aa 1-4281 and 4871-4967), six transmembrane domains, and one pore-forming domain (aa 4820-4829). It is the predominant form in cardiac muscle and is also expressed in Purkinje cells of cerebellum and cerebral cortex. It plays a key role in triggering cardiac muscle contraction and aberrant channel activation can lead to cardiac arrhythmia. Channel activity is modulated by ryanodine that binds to the open Ca2+-release channel with high affinity. At low concentrations, ryanodine maintains the channel in an open conformation, but high concentrations of ryanodine inhibit channel activity. Channel activity is also modulated by phosphorylation. Phosphorylation at serine 2808 and serine 2814 is shown to increase the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks, and increased cytoplasmic Ca2+ levels. (Ref.: Xiao, Z., et al. (2016). J. Biol. Chem. 291(47); 24528-24537; Lanner, JT., et al. (2010). Cold Spring Harb. Perspect. Biol. 2(11); a003996).

特異性

This rabbit polyclonal antibody detects Ryanodine receptor 2 (RyR2) phosphorylated on serine 2808.

免疫原

A KLH-conjugated linear peptide corresponding to 8 amino acids surrounding phosphoserine 2808 from human Ryanodine receptor 2.

應用

Quality Control Testing

Evaluated by Western Blotting in rabbit skeleton muscle microsomal preparation.

Western Blotting Analysis (WB): A 1:300 dilution of this antibody detected RyR2 phosphorylated on serine 2808 in rabbit skeleton muscle microsomal preparations, but not in microsomes treated with protein phosphatase.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

外觀

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without azide

重構

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable