ABS218

Anti-phospho-DBC1 (Thr454) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: DBIRD complex subunit KIAA1967, Deleted in breast cancer gene 1 protein, DBC-1, DBC.1, p30 DBC
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_066997
• UniProt登錄號: Q8N163
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pThr454)
• 基因資訊: human ... BRINP1(1620)

描述

一般說明

Deleted in breast cancer gene 1 protein (DBC1) is a large, nuclear protein with multiple functions in cell survival that binds directly to the estrogen receptor α (ERα) hormone-binding domain in a strict ligand-independent manner. DBC1 promotes p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity. It is ubiquitously expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.

特異性

This antibody recognizes DBC1 phosphorylated at Thr454.

免疫原

Epitope: Phosphorylated Thr454

KLH-conjugated linear peptide corresponding to human DBC-1 phosphorylated at Thr454.

應用

Anti-phospho-DBC1 (Thr454) Antibody is an antibody against phospho-DBC1 (Thr454) for use in Western Blotting, Peptide Inhibition Assay.

Peptide Inhibition Assay: A 1:500 dilution from a representative lot blocked DBC-1 phosphorylated at Thr454 in untreated and etoposide treated Jurkat cell lysates.

Research Category
Signaling

Research Sub Category
Developmental Signaling

品質

Evaluated by Western Blot in untreated and etoposide treated Jurkat cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected DBC-1 phosphorylated at Thr454 in 10 µg of etoposide treated Jurkat cell lysates.

標靶描述

~130 kDa observed. Uniprot states the calculated molecular weight is 103 kDa but has been shown at ~130kDa (Satoh, M., et al. (1995). Brain Res Dev Brain Res. 87(2):111-119).

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Untreated and etoposide treated Jurkat cell lysates

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable