ABE434

Anti-TIF1-alpha Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, Tripartite motif-containing protein 24
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, human
• 物種活性（以同源性預測）: rhesus macaque (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_056989
• UniProt登錄號: O15164
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... TRIM24(8805)

描述

一般說明

Transcription intermediary factor 1-alpha (TIF1-alpha) is also called E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, and Tripartite motif-containing protein 24. TIF1-alpha is a transcriptional coactivator that interacts with chromatin depending on histone H3 modifications, with the highest affinity for histone H3 that is both unmodified at ′Lys-4′ (H3K4me0) and acetylated at ′Lys-23′ (H3K23ac). TIF1-alpha has E3 protein-ubiquitin ligase activity, helps regulate cell proliferation and apoptosis and up-regulates transcription activation. Aberrant TIF1-alpha can cause thyroid papillary carcinoma (TPC) and is up-regulated in some breast cancers.

特異性

Other homologies: Rat (93% sequence homology).

This antibody recognizes the C-terminus of the Nuclear Receptor Binding Site (NRBS) of TIF1-alpha.

免疫原

Epitope: C-terminus of the Nuclear Receptor Binding Site (NRBS)

KLH-conjugated linear peptide corresponding to the C-terminus of the Nuclear Receptor Binding Site (NRBS) of human TIF1-alpha.

應用

Detect TIF1α using this rabbit polyclonal antibody, Anti-TIF1-alpha Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: A 1:50 dilution from a represenative lot detected TIF1-alpha in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected TIF1-alpha in 10 µg of HeLa cell lysate.

標靶描述

~140 kDa observed. Uniprot descrives 2 isoforms at ~117 kDa and ~113 kDa due to alternative splicing. This protein has been observed at ~140 kDa (Gunawardena, H., et al. (2008). Rheumatology (Oxford). 47(3):324-328.).

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
HeLa cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1