ABE1342

Anti-ZBTB4 (KAISO-L1)

from rabbit

• 同義詞: Zinc finger and BTB domain-containing protein 4, KAISO-like zinc finger protein 1, KAISO-L1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 物種活性（以同源性預測）: monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 包裝: antibody small pack of 25 μg
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_001122305
• UniProt登錄號: Q9P1Z0
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZBTB4(57659)

描述

一般說明

Zinc finger and BTB domain-containing protein 4 (UniProt: Q9P1Z0; also known as KAISO-like zinc finger protein 1, KAISO-L1, ZBTB4) is encoded by the ZBTB4 (also known as KIAA1538) gene (Gene ID: 57659) in human. ZBTB4 is an epigenetic regulator that localizes at pericentromeric heterochromatin. It is shown to be an essential component in maintaining genomic stability in mammals. It serves as a transcriptional repressor with bimodal DNA-binding specificity. It represses transcription in a methyl-CpG-dependent manner and binds with a higher affinity to methylated CpG dinucleotides in the consensus sequence 5′-CGCG-3′, but can also bind to the non-methylated consensus sequence 5′-CTGCNA-3′ (also known as the consensus kaiso binding site). ZBTB4 contains six C2H2-type zinc-finger domains and two proline-rich regions (aa 435-536 and 610-654). Its BTB (Broad-Complex, Tramtrack and Bric a brac) domain is localized in the amino acids 30-152. It is shown to be is frequently downregulated in cancer. ZBTB4 is phosphorylated by HIPK2 (Homeodomain Interacting Protein Kinase 2) and phosphorylation reduces its stability and triggers its degradation in response to DNA damage. (Ref.: Roussel-Gervais A., et al. (2017). Cancer Res; 77(1); 62-73; Yamada, D., et al (2009). Oncogene. 28(27); 2535-2544).

特異性

This rabbit polyclonal antibody detects Zinc finger and BTB domain-containing protein 4 (KAISO-L1) in human cells. It targets an epitope within 17 amino acids from the internal region.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the internal region of human Zinc finger and BTB domain-containing protein 4.

應用

Anti-ZBTB4 (KAISO-L1), Cat. No. ABE1342, is a highly specific rabbit polyclonal antibody that targets Zinc finger and BTB domain-containing protein 4 and has been tested for use in Immunohistochemistry (Paraffin) and in Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ZBTB4 (KAISO-L1) in human fallopian tube and human cervical cancer tissue sections.

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ZBTB4 (KAISO-L1) in PC3 cell lysate.

標靶描述

~105 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable