ABE1320

Anti-ASXL2

from rabbit

• 同義詞: Putative Polycomb group protein ASXL2, Additional sex combs-like protein 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_060733
• UniProt登錄號: Q76L83
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ASXL2(55252)

描述

一般說明

Putative Polycomb group protein ASXL2 (UniProt: Q76L83; also known as Additional sex combs-like protein 2) is encoded by the ASXL2 (also known as ASXH2, KIAA1685) gene (Gene ID: 55252) in human. ASXL2 is a putative Polycomb group (PcG) protein that acts by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. It is not required to initiate repression, but is essential to maintain it during later stages of development. They probably act via methylation of histones, rendering chromatin heritably changed in its expressibility. ASXL2 is shown to be involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as peroxisome proliferator-activated receptor gamma (PPARG). It also acts as coactivator for PPARG and enhances its adipocyte differentiation-inducing activity; the function seems to involve differential recruitment of acetylated and methylated histone H3. ASXL2 is reported to be essential for haematopoiesis and acts as a haploinsufficient tumor suppressor in leukemia. ASXL2 gene is one of most commonly mutated genes in AML patients bearing the AML1-ETO fusion oncoprotein and loss of ASXL2 is shown to promote AML1-ETO-mediated leukemogenesis. ASXL2 protein contains two LXXLL motifs (aa 303-307 and 887-891), which are required for an association with nuclear receptors. It also contains an atypical PHD-type zinc finger regions (aa 1397-1434). Two isoforms of ASXL2 are reported that are produced by alternative splicing. Mutation in ASXL2 gene have also been linked to the development of Shashi-Pena syndrome that is characterized by delayed psychomotor development, intellectual disability, and macrocephaly, and neonatal feeding difficulties. (Ref.: Micol, JB et al. (2017). Nat. Commun. DOI: 10.1038/ncomms15429).

特異性

This rabbit polyclonal antibody detects Additional sex combs-like protein 2 (ASXL2) in human cells. It targets an epitope within 22 amino acids from the internal region.

免疫原

Epitope: unknown

KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human Additional sex combs-like protein 2 (ASXL2).

應用

Anti-ASXL2, Cat. No. ABE1320, is a highly specific rabbit polyclonal antibody that targets Additional sex combs-like protein 2 and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected ASXL2 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in Raji nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected ASXL2 in 10 µg of Raji nuclear extract.

標靶描述

~154 kDa observed; 153.82 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable