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AB5675

Sigma-Aldrich

抗代谢型谷氨酸受体5抗体,疼痛

Chemicon®, from rabbit

同義詞:

Metabotropic glutamate receptor 5, Metabotropic Glutamate Receptor 5 Antibody, mGluR5, Anti-mGluR5

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

rat, mouse

物種活性(以同源性預測)

human (based on 100% sequence homology), bovine (based on 100% sequence homology), primate (based on 100% sequence homology), horse (based on 100% sequence homology), feline (based on 100% sequence homology)

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... GRM5(2915)

一般說明

代谢型谷氨酸受体5或mGluR5(UniProt Q3UVX5;也称为G蛋白偶联受体GRM5,G蛋白偶联受体家族C,第1组,成员E)由鼠种中的Grm5(也称为Gprc1e,Mglur5,AI850523、6430542K11Rik)基因编码(基因ID 108071)。代谢型谷氨酸受体属于G蛋白偶联受体家族,根据其序列同源性,推定的信号转导机制和药理特性将其分为3类。mGluR1和mGluR5构成I类代谢型谷氨酸受体。在神经元和小胶质细胞的突触后末端发现了mGluR5。mGluR5通过Gq/G11偶联发出信号以激活磷脂酶C,从而导致钙动员和蛋白激酶C(PKC)激活。此外,据报告,mGluR5还以不依赖G蛋白的方式介导涉及Src家族蛋白酪氨酸激酶的信号传导。

特異性

该抗体不能检测亚型2。

免疫原

KLH偶联的线性肽,对应于大鼠代谢型谷氨酸受体5的细胞质域。

應用

抗代谢型谷氨酸受体5抗体,疼痛是用于IH &WB的一种针对代谢型谷氨酸受体5的抗体。
研究子类别
神经递质& 受体
研究类别
神经科学
蛋白印迹分析: 0.1-0.5 μg/mL,大鼠脑微粒体制备使用ECL。与~132 kDa的条带反应。
免疫组化:在用含15%饱和苦味酸的4%多聚甲醛的0.1 M PB、pH值7.4固定的大鼠脑和小鼠脑切片上为0.1-0.2 μg/ml。
免疫组化分析:代表性批次的1:50稀释液在小鼠后脑和大鼠结肠组织中检测到代谢型谷氨酸受体5。

品質

通过蛋白质印迹对大鼠脑组织裂解物的微粒体制剂进行了评估。

蛋白质印迹分析:2.0 µg/mL 的该抗体在 10 µg大鼠脑组织裂解物的微粒体制剂中检测到代谢型谷氨酸受体5。

標靶描述

观察值〜150 kDa。某些裂解液可能会出现未表征的条带。

聯結

替代品:AB1596;06-451

外觀

免疫亲和纯化
纯化的兔多克隆抗体,溶于含0.05%叠氮化钠的PBS缓冲液中

儲存和穩定性

自收到之日起,在 2-8°C 条件下可稳定保存1年

分析報告

对照
脑组织

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Pratyush S Suryavanshi et al.
Molecular pharmacology, 90(2), 96-105 (2016-05-28)
The delta family of ionotropic glutamate receptors consists of glutamate delta-1 (GluD1) and glutamate delta-2 receptors. We have previously shown that GluD1 knockout mice exhibit features of developmental delay, including impaired spine pruning and switch in the N-methyl-D-aspartate receptor subunit
Kalina Andreeva et al.
Ophthalmology and eye diseases, 6, 43-54 (2014-09-12)
Ischemia/reperfusion (IR) injury has been associated with several retinal pathologies, and a few genes/gene products have been linked to IR injury. However, the big picture of temporal changes, regarding the affected gene networks, pathways, and processes remains to be determined.
Rocco G Gogliotti et al.
Human molecular genetics, 25(10), 1990-2004 (2016-03-05)
Rett syndrome (RS) is a neurodevelopmental disorder that shares many symptomatic and pathological commonalities with idiopathic autism. Alterations in protein synthesis-dependent synaptic plasticity (PSDSP) are a hallmark of a number of syndromic forms of autism; in the present work, we
Dany Arsenault et al.
PloS one, 10(11), e0142093-e0142093 (2015-11-05)
Parental microglial induced neuroinflammation, triggered by bacterial- or viral infections, can induce neuropsychiatric disorders like schizophrenia and autism to offspring in animal models. Recent investigations suggest that microglia, the resident immune cells of the brain, provides a link between neurotransmission
Anthony D Umpierre et al.
Experimental neurology, 279, 116-126 (2016-02-21)
More efficient or translationally relevant approaches are needed to model acquired temporal lobe epilepsy (TLE) in genetically tractable mice. The high costs associated with breeding and maintaining transgenic, knock-in, or knock-out lines place a high value on the efficiency of

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