推薦產品
生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
polyclonal
純化經由
affinity chromatography
物種活性
rat, mouse
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CHRM1(1128)
特異性
Recognizes a full length m1 protein. It has exhibited no cross reactivity with other muscarinic proteins tested so far.
免疫原
GST fusion protein and part of i3 intercellular loop of human m1 muscarinic acetylcholine receptor (amino acids 227-353) (Accession P11229).
應用
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Muscarinic Acetylcholine Receptor m1 Antibody is validated for use in IH, WB for the detection of Muscarinic Acetylcholine Receptor m1.
Western blot: 1:200 using ECL on rat brain membranes with proteinase inhibitors. M1 is approximately 60-80kDa in size, some preparations yield 125kDa dimers (photo). Heating samples to 80-90C rather than fulling boiling and adjusting sample pH to neutral can help in preventing dimer formation.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Immunohistochemistry: rat frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only.
Optimal working dilutions must be determined by the end user.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Immunohistochemistry: rat frozen sections. 1:50-1:200, internal epitope; use triton X-100 in blocking buffer only; dilute primary antibody in PBS or TBS with 0.5%-1% BSA or NGS only.
Optimal working dilutions must be determined by the end user.
外觀
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
儲存和穩定性
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
分析報告
Control
Included free of charge with the antibody is 60 μg of control fusion protein (lyophilized powder). The stock solution of the fusion protein can be made up using 100 μL of PBS. For positive control, in Western blot using 10 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Included free of charge with the antibody is 60 μg of control fusion protein (lyophilized powder). The stock solution of the fusion protein can be made up using 100 μL of PBS. For positive control, in Western blot using 10 ng of protein per minigel lane. For negative control, preincubate 3 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
M Takamori et al.
European journal of neurology, 14(11), 1230-1235 (2007-09-04)
The Lambert-Eaton myasthenic syndrome (LEMS), often associated with small-cell lung carcinoma (SCLC), is a disorder of acetylcholine (ACh) release from motor nerve terminals. In most patients, it is caused by autoantibodies against the P/Q-type voltage-gated calcium channels (VGCC) that trigger
Marci L Smith et al.
Molecular vision, 20, 1328-1356 (2014-10-30)
The α7 nicotinic acetylcholine receptor (nAChR) is widely expressed in the nervous system, including in the inner retinal neurons in all species studied to date. Although reductions in the expression of α7 nAChRs are thought to contribute to the memory
Gitte Jositsch et al.
Naunyn-Schmiedeberg's archives of pharmacology, 379(4), 389-395 (2008-11-01)
Acetylcholine (ACh) is a major regulator of visceral function exerting pharmacologically relevant effects upon smooth muscle tone and epithelial function via five types of muscarinic receptors (M1R-M5R). In this paper, we assessed the specificity of muscarinic receptor (MR) antibodies in
Chase M Carver et al.
Frontiers in neuroscience, 15, 681144-681144 (2021-09-08)
We investigated the contribution of excitatory transient receptor potential canonical (TRPC) cation channels to posttraumatic hyperexcitability in the brain 7 days following controlled cortical impact model of traumatic brain injury (TBI) to the parietal cortex in male adult mice. We
Eleanor D Muise et al.
Data in brief, 10, 482-486 (2017-01-06)
The data presented in this article are related to the research article entitled "Distribution of muscarinic acetylcholine receptor subtypes in the murine small intestine" (E.D. Muise, N. Gandotra, J.J. Tackett, M.C. Bamdad, R.A. Cowles, 2016) [1]. We recently demonstrated that
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