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AB5016

Sigma-Aldrich

Anti-GABA (No Gluteraldehyde) Antibody

Chemicon®, from rabbit

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

rat

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

特異性

GABA (gamma-aminobutyrate). The antibody has been calibrated against a spectrum of antigens to assure hapten selectivity and proper affinity. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine, 1-amino-4-guanidobutane (AGB), D/L-arganine, D/L-aspartate, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, L-threonine, L-tryptophan, L-tyrosine.

免疫原

GABA-glutaraldehyde

應用

Immunohistochemistry using silver-intensified immunogold or fluorescence (see recommended protocol). Samples should be fixed with 0.05% - 2.5% glutaraldehyde for optimum detection.

*This antibody has also been used and found to work with a zero-low glutaraldehyde / high paraformaldehyde fixation (4% paraformaldehyde in 0.1M phosphate buffer / 3% sucrose fixative ). The minimum glutaraldehyde concentration for AB5016 is 0.00%. See protocol that follows. Performance is good with frozen sections, Vibratome sections and tissue culture formats, when penetrating reagents such as 0.3% Triton X-100 are used.

Optimal working dilutions must be determined by the end user.

DILUTION: Prepare enough of the AB5016 for your days use by diluting 100X with 1% GSPBT.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
This Anti-GABA (No Gluteraldehyde) Antibody is validated for use in IH for the detection of GABA (No Gluteraldehyde).

外觀

Format: Purified
IgG fraction in sterile 0.1M phosphate buffer. No preservative

儲存和穩定性

Maintain stock at 2-8°C in undiluted aliquots for up to 6 months. This stock is extremely stable under normal use and routine storage at 2-8°C. Do not freeze this stock.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Insu Kwon et al.
Physical activity and nutrition, 27(2), 39-49 (2023-08-16)
Aging is closely associated with chronic metabolic diseases, such as obesity, which lead to increased adiposity, skeletal muscle wasting, and imbalanced cellular energy metabolism. However, transcriptional profiles representing energy imbalances in aging-induced obesity are not fully understood. Thus, this study
Matthias Braun et al.
The Journal of general physiology, 123(3), 191-204 (2004-02-11)
We have explored whether gamma-aminobutyric acid (GABA) is released by regulated exocytosis of GABA-containing synaptic-like microvesicles (SLMVs) in insulin-releasing rat pancreatic beta-cells. To this end, beta-cells were engineered to express GABA(A)-receptor Cl(-)-channels at high density using adenoviral infection. Electron microscopy
Haijie Yu et al.
Journal of pineal research, 61(1), 69-81 (2016-03-29)
Pinealocytes secrete melatonin at night in response to norepinephrine released from sympathetic nerve terminals in the pineal gland. The gland also contains many other neurotransmitters whose cellular disposition, activity, and relevance to pineal function are not understood. Here, we clarify
Neurotransmitter synthesis in poststroke cortical neurogenesis in adult rats.
Gu, W; Gu, C; Jiang, W; Wester, P
Stem Cell Research null
Age-dependent disease expression determines remodeling of the retinal mosaic in carriers of RPGR exon ORF15 mutations.
Beltran, WA; Acland, GM; Aguirre, GD
Investigative Ophthalmology & Visual Science null

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