676602

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

lyophilized, clone 16C2, Calbiochem^®

• 同義詞: Anti-Vasodilator-stimulated Phosphoprotein, Activated
• 分類程式碼代碼: 12352203

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 16C2, monoclonal
• 形狀: lyophilized
• 包含: ≤0.1% sodium azide as preservative
• 物種活性: mouse, human
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 同型: IgG1
• 運輸包裝: ambient
• 儲存溫度: −20°C
• 目標翻譯後修改: phosphorylation (pSer239/pSer239)

描述

一般說明

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser²³⁹.

This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

免疫原

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH

應用

ELISA (0.05 µg/ml)

Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

警告

Toxicity: Standard Handling (A)

外觀

Lyophilized from 2X PBS, PEG, sucrose.

重構

Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

分析報告

Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets

其他說明

Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser²³⁹ as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton^™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.

Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable