524715

Phosphodiesterase, Type V, cGMP-Specific, Bovine, Recombinant, Spodoptera frugiperda

• 同義詞: PDE V, Phosphodiesterase V, cGMP-binding cGMP-specific PDE
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 形狀: liquid
• 品質等級: 100
• 比活性: ≥50,000 units/mL
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

Recombinant, bovine c-GMP-specific phosphodiesterase expressed in S. frugiperda insect cells. Supplied as partially purified, catalytically active, dimeric holoenzyme. A target enzyme for Viagra (sildenafil). Suitable for enzymatic studies and as a standard in Western blots using Anti-Phosphodiesterase V Antibody (Cat. No. 524583). For enzymatic studies a dilution of 1:1000 recommended. For use as a immunoblot standard 2 to 3 µl per lane is recommended. In immunoblotting, gives band at ~92 kDa.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will hydrolyze 1.0 pmol cGMP per min at 25°C, pH 7.2.

外觀

In sodium phosphate buffer, 20% glycerol, pH 7.2.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Webb, D.J., et al. 1999. Am. J. Cardiol. 83, 21C.
Turko, I.V., et al. 1998. J. Biol. Chem. 273, 6460.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1