481974

NADPH-P450 Oxidoreductase, Human, Recombinant, S. frugiperda

• 酶委員會編號: 1.6.2.4 (BRENDA, IUBMB)
• 分類程式碼代碼: 12352202

屬性

• 化驗: ≥90% (SDS-PAGE)
• 品質等級: 100
• 形狀: liquid
• 比活性: ≥20 units/mg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 溶解度: aqueous buffer: soluble
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratio 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.

Recombinant, human NADPH-P450 oxidoreductase expressed in S. frugiperda insect cells. A membrane-bound flavoprotein containing one mole each of FAD and FMN. Transfers electrons from NADPH to the various isoforms of cytochrome P450. Typically used at ratios ranging from 1:10 to 1:2 (reductase:P450) for in vitro reconstitution assays. Suitable for in vitro drug metabolism and toxicology research including reconstituted P450-dependent biotransformation reactions.

包裝

Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will oxidize 1 µmol of cytochrome c per min at 20°C, pH 7.7.

外觀

In 10 mM potassium phosphate buffer, 1 mM DTT, 100 µM EDTA, 20% glycerol, pH 7.7.

重構

Following initial thaw, aliquot and freeze (-70°C).

其他說明

Shen, A.L., et al. 1989. J. Biol. Chem. 264, 7584.
Yamano, S., et al. 1989. Mol. Pharmacol.36, 83.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10-13 - German Storage Class 10 to 13