262015

DNA Base Excision Repair Pathway Inhibitor

The DNA Base Excision Repair Pathway Inhibitor, also referenced under CAS 6960-45-8, controls the biological activity of APE1 (human apurinic/apyrimidinic endonuclease.

• 同義詞: DNA Base Excision Repair Pathway Inhibitor, 7-Nitroindole-2-carboxylic acid, CRT0044876
• 經驗公式（希爾表示法）: C₉H₆N₂O₄
• CAS號碼: 6960-45-8
• 分子量：: 206.15
• MDL號碼: MFCD00044720
• 分類程式碼代碼: 12352200
• NACRES: NA.54

屬性

• 品質等級: 100
• 化驗: ≥98% (HPLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; protect from light
• 顏色: tan
• 溶解度: DMSO: 5 mg/mL
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• InChI: 1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1
• InChI 密鑰: BIUCOFQROHIAEO-UHFFFAOYSA-M

描述

一般說明

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.

A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC₅₀ ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H₂O₂ in HeLa, HT1080, and MDA-MB-231 cells.

生化／生理作用

Cell permeable: yes

Primary Target
APE 1

Product does not compete with ATP.

Reversible: no

Target IC₅₀: ~3 µM against APE1 (human apurinic/apyrimidinic endonuclease

包裝

Packaged under inert gas

警告

Toxicity: Irritant (B)

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Madhusudan, S., et al. 2005. Nucl. Acids Res.33, 4711.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H319,H334
• 防範說明: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• 危險分類: Eye Irrit. 2 - Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable