218725

Caspase-5, Human, Recombinant, E. coli

Caspase-5, Human, Recombinant, is expressed in E. coli. It is an inflammatory caspase.

• 同義詞: ICErel-III, TY
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 100
• 化驗: ≥80% (SDS-PAGE)
• 形狀: liquid
• 比活性: ≥100,000 units/mg protein
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 運輸包裝: wet ice
• 儲存溫度: −70°C

描述

一般說明

M.W. 10,000 and 22,000 (heterodimer).

Recombinant, human caspase-5 expressed in E. coli. A member of the caspase-1 subfamily of cysteine proteases involved in Fas-mediated apoptosis. Caspase-5 cleavage site is reported to be Asp³¹¹ and Asp³³⁰. May also cleave at Asp¹²¹. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

Recombinant, human caspase-5 expressed in E. coli. Suitable for the study of enzyme regulation and kinetics, target substrate cleavage, and inhibitor screening. MW: 10000 and 22000.

警告

Toxicity: Standard Handling (A)

單位定義

One unit is defined as the amount of enzyme that will cleave 1.0 pmol substrate Ac-LEHD-pNA (Cat. No. 218805) per min at 25°C, pH 7.4.

外觀

In 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.

重構

After initial defrost, aliquot and freeze at -70°C.

其他說明

Schwartz, S. et al. 1999. Cancer Res. 59, 2995.
Fucheu, C., et al. 1996. Eur. J. Biochem. 236, 207.
Munday, N.A., et al. 1995. J. Biol. Chem. 270, 15870.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2