14-758-M

Ret (V804L) Protein, active, 10 µg

Active, N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation. For use in Kinase Assays.

• 分類程式碼代碼: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

屬性

• 生物源: human
• 品質等級: 100; 300
• 重組細胞: expressed in baculovirus infected Sf21 cells
• 分子量: M_w 79.2 kDa
• 製造商／商標名: Upstate^®
• 技術: activity assay: suitable (kinase)
• NCBI登錄號: NM_000323
• UniProt登錄號: P07949; P35546 (mouse)
• 基因資訊: human ... RET(5979)

描述

一般說明

N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804L mutation.

Product Source: Expressed by baculovirus in Sf21 insect cells.

The V804L mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Lombardo F. et al., (2002), JCEM, 87, 1674-1680; Lesueur F. et al, (2005), JCEM, 90, 3454-3457).

應用

Research Category
Inflammation & Immunology

生化／生理作用

Protein Target: Ret

Target Sub-Family: TK

包裝

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-758M when ordering the 250 µg size.

品質

Routinely evaluated by phosphorylation of CHKtide.

外觀

Glutathione agarose affinity chromatography

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

儲存和穩定性

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable