1.16886

Fractogel EMD COO⁻ (M)

• 同義詞: Fractogel^® EMD COO^- (M)
• 分類程式碼代碼: 41115711

屬性

• ligand: carboxymethyl
• 品質等級: 400
• 描述: weak cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
• 無菌: sterile (Caustic Stable)
• 產品線: Fractogel^®
• 形狀: resin
• 特定分析物: MAbs
• 參數: 300 cm/hr flow rate; 8 bar max. pressure
• 基質活性組: methacrylate
• 平均粒徑: 40-90 μm
• 容量: 100 mg binding capacity (lysozyme/ml of resin)
• 轉變溫度: flash point 35 °C (calculated)
• 密度: 1.43 g/cm³ at 20 °C
• 應用: recombinant protein; vaccine development
• 分離技術: cation exchange; weak cation exchange
• 儲存溫度: 2-30°C

描述

一般說明

Weak cation exchange Fractogel^® resin featuring the tentacle technology, efficient for difficult aggregate removal

特點和優勢

Fractogel^® EMD COO^- (M) enables:

• Good resolution between monomers and aggregates
• Efficient aggregate and HCP removal combined with high yield
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD COO^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

包裝

• 1.16886.0100: Fractogel^® EMD COO- (M) Resin 100ml
• 1.16886.0010: Fractogel^® EMD COO- (M) Resin 10ml
• 1.16886.0500: Fractogel^® EMD COO- (M) Resin 500ml
• 1.16886.5000: Fractogel^® EMD COO- (M) Resin 5L

Plastic bottle

分析報告

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates,no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 10 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 80 - 120 mg/ml
Functional test (b:a): ≤ 0.30
Functional test (c:d): ≤ 0.70
Functional test: Separation of chymotrypsinogen A, cytochrome C and lysozyme

法律資訊

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P210 - P233 - P240 - P241 - P242 - P243
• 危險分類: Flam. Liq. 3
• 儲存類別代碼: 3 - Flammable liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 95.0 °F
• 閃點（°C）: 35 °C