09-142

Anti-phospho-ACK1 (Tyr284) Antibody

Upstate^®, from rabbit

• 同義詞: TNK2, tyrosine kinase non-receptor 2, p21cdc42Hs, activated Cdc42-associated kinase 1, activated p21cdc42Hs kinase
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity purified immunoglobulin
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 製造商／商標名: Upstate^®
• 技術: dot blot: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NM_001010938.1; NM_005781.4
• UniProt登錄號: Q07912
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pTyr284)
• 基因資訊: human ... TNK2(10188)

描述

一般說明

Activated CDC42 kinase 1 (UniProt: Q07912; also known as ACK-1, Tyrosine kinase non-receptor protein 2) is encoded by the TNK2 (also known as ACK1) gene (Gene ID: 10188) in human. ACK-1 is a non-receptor tyrosine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth, and proliferation. It transduces extracellular signals to cytosolic and nuclear effectors. It is known to phosphorylate AKT1 (on tyrosine 176), androgen receptor (AR; on tyrosine 267), and WWOX (on tyrosine 287). It is also reported to phosphorylate MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. It has also been implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. It binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Its protein kinase domain is localized in amino acids 126-385 and its SH3 domain resides in amino acids 388-448. It also contains a SAM-like domain that is reported to be essential for NEDD4-mediated ubiquitination and promotes its membrane localization and dimerization to allow for autophosphorylation . It undergoes phosphorylation on tyrosine 284 and the phosphorylated form shows a significant increase in expression in prostate cancer breast cancers during progressive stages. (Ref.: Mahajan, K., et al. (2010). PLoS One. 5(3); e9646; Prieto-Echagüe, V., et al. (2010). BMC Biochem. 22; 42; van der Horst, E., et al. (2005). Proc. Natl. Acad. Sci. USA. 102(44); 15901-15906).

特異性

This rabbit polyclonal antibody detects Activated CDC42 kinase 1 (ACK1) phosphorylated on tyrosine 284.

免疫原

KLH-conjugated linear peptide corresponding to 9 amino acids surrounding human phospho ACK1 (Y284).

應用

Anti-phospho-ACK1 (Tyr284), Cat. No. 09-142, is a rabbit polyclonal antibody that detects ACK1 phosphorylated on tyrosine 284 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.




Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.Western Blotting Analysis: A 1:250 dilution of this antibody detected phospho-ACK1(Tyr284) in A431 cell lysate.


Tested ApplicationsDot Blot Analysis: Anti-phospho-ACK1 (Tyr284)) antibody 09-142 detected Phospho-ACK1 (Tyr284) peptide, but not the unmodified ACK1 peptide.Peptide Inhibition Assay: Target band detection in a lysate from A431 cells was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

品質

Routinely evaluated by immunoblot.

標靶描述

~160 kDa observed; 114.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Purified rabbit polyclonal antibody in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl with 0.07% sodium azide with 30% glycerol.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2