07-2270

Anti-WNK4 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Serine/threonine-protein kinase WNK4, Protein kinase lysine-deficient 4, Protein kinase with no lysine 4
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, mouse
• 物種活性（以同源性預測）: rat (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登錄號: NP_115763
• UniProt登錄號: Q96J92
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... WNK4(65266)

描述

一般說明

WNK4 is a serine-threonine protein kinase that is commonly expressed in the distal nephron. Mutations in WNK1 and WNK4 cause pseudohypoaldosteronism type II, which causes hypertension, hyperkalemia, hyperchloremia and metabolic acidosis. WNK4 has been shown to decrease Na-Cl cotransporter activity and the potassium channel by removing it from the cell surface. It is thought that activating WNK4 inhibits the activity of WNK1. WNK4 has been shown to play a role in regulating blood pressure in addition to potassium homeostasis.

特異性

This antibody recognizes WNK4 at the C-terminus.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human WNK4 at the C-terminus.

應用

Immunohistochemistry Analysis: 1:1,000 dilution from a previous lot detected WNK4 in normal human kidney tissue.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This WNK4 antibody is validated for use in WB & IHC for the detection of the WNK4 protein.

品質

Evaluated by Western Blot in mouse kidney cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected WNK4 on 10 µg of mouse kidney cell lysate.

標靶描述

~160 kDa observed. This protein has 3 isoforms produced by alternative splicing. Hengl, T., et al. (2010). PNAS. 107(13):6052–6057.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse kidney cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable