07-1596
Anti-SIRT3 (CT) Antibody
from rabbit
同義詞:
SIR2-like protein 3 , mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物源
rabbit
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human, mouse
技術
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... SIRT3(23410)
mouse ... Sirt3(64384)
一般說明
SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70.
特異性
Not tested in other species.
This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells.
免疫原
Epitope: C-terminus
The immunogen was a peptide corresponding the C-terminal region of human SIRT3.
應用
Anti-SIRT3 (C-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of SIRT3 (C-terminus) also known sirtuin 3 & has been validated in WB, ELISA & IHC.
ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).
Immunohistochemistry:
This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins
Histone Modifying Proteins
品質
Western Blot Analysis:
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate.
標靶描述
44 kDa
外觀
Purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.09% NaN3.
儲存和穩定性
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
C2C12 cell lysate
C2C12 cell lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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Frontiers in cellular neuroscience, 10, 73-73 (2016-04-06)
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