推薦產品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
B-6-4, monoclonal
物種活性
human, rabbit, rat, bovine, mouse
製造商／商標名
Upstate®
濃度
1 mg/mL
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PLCG1(5335)
特異性
PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins
免疫原
Purified soluble phospholipase C (PLC) g-1 from bovine brain
應用
Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Lipid Signaling
Lipid Signaling
品質
Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.
標靶描述
~135 kDa
外觀
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
分析報告
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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訊號詞
Warning
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.
Roli Prasad, Jayashree Venkatasubramanian, Milen Amde, Mrinalini Chatta Rao
Digestive Diseases and Sciences null
Aging and a peripheral immune challenge interact to reduce mature brain-derived neurotrophic factor and activation of TrkB, PLCgamma1, and ERK in hippocampal synaptoneurosomes.
Cortese, GP; Barrientos, RM; Maier, SF; Patterson, SL
The Journal of Neuroscience null
T Fukazawa et al.
The Journal of biological chemistry, 270(34), 20177-20182 (1995-08-25)
Tyrosine phosphorylation of cellular proteins is an early and an essential step in T cell receptor-mediated lymphocyte activation. Tyrosine phosphorylation of transmembrane receptor chains (such as zeta and CD3 chains) and membrane-associated proteins provides docking sites for SH2 domains of
L Rui et al.
The Journal of biological chemistry, 274(15), 10590-10594 (1999-04-03)
Nerve growth factor (NGF) is essential for the development and survival of sympathetic and sensory neurons. NGF binds to TrkA, activates the intrinsic kinase activity of TrkA, and promotes the differentiation of pheochromocytoma (PC12) cells into sympathetic-like neurons. Several signaling
C K Miranti et al.
The Journal of biological chemistry, 274(15), 10571-10581 (1999-04-03)
Adhesion of fibroblasts to extracellular matrices via integrin receptors is accompanied by extensive cytoskeletal rearrangements and intracellular signaling events. The protein kinase C (PKC) family of serine/threonine kinases has been implicated in several integrin-mediated events including focal adhesion formation, cell
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