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重要文件

200964

Sigma-Aldrich

赤藓红

Dye content ≥95 %

同義詞:

溶剂红 140

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About This Item

經驗公式(希爾表示法):
C20H8I4O5
CAS號碼:
分子量::
835.89
顏色索引編號:
45430:2
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
暫時無法取得訂價和供貨情況

形狀

powder

成份

Dye content, ≥95%

技術

microbe id | staining: suitable

顏色

orange to red-orange

mp

303 °C (dec.) (lit.)

溶解度

1 M NH4OH: 1 mg/mL
1 M NH4OH: soluble 1 mg/mL, orange to red

&epsilon ;(消光係數)

≥102000 at 526-534 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥13000 at 310-318 nm in 1 M NH4OH at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)
≥32000 at 258-266 nm at 0.005 g/L (95% Methanol 5% 0.1M Na2CO3)

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

Oc1c(I)cc2c(Oc3c(I)c(O)c(I)cc3C24OC(=O)c5ccccc45)c1I

InChI

1S/C20H8I4O5/c21-11-5-9-17(13(23)15(11)25)28-18-10(6-12(22)16(26)14(18)24)20(9)8-4-2-1-3-7(8)19(27)29-20/h1-6,25-26H

InChI 密鑰

OALHHIHQOFIMEF-UHFFFAOYSA-N

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應用

赤藓糖苷 B 已被用作荧光寿命成像的染料。[1][2]

生化/生理作用

赤藓红 B,也称为曙红 B,是一种蒽染料。[3]它在抑制大鼠尾状核突触体中的多巴胺摄取和高亲和力3H-哇巴因结合和离子转运中起主要作用。[4][5]

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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Erythrosin B is a specific inhibitor of high affinity 3H-ouabain binding and ion transport in rat brain
Silbergeld E K
Neuropharmacology, 20, 87-90 (1981)
Practical and reliable FRET/FLIM pair of fluorescent proteins.
Shcherbo D
BMC biotechnology, 2009-2009 null
Bright monomeric red fluorescent protein with an extended
fluorescence lifetime
Merzlyak EM
Nature Methods, 4, 555-557 (2007)
Erythrosin B inhibits dopamine transport in rat caudate synaptosomes.
Lafferman JA and Silbergeld EK
Science, 205, :410-:412 (1979)
Zhen Cheng et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 201, 281-287 (2018-05-15)
Because of the serious harm to animals and the environment associated with perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), a rapid, sensitive and low-cost method for detecting PFOS and PFOA is of great importance. In this paper, a novel sensing

Questions

1–2 of 2 Questions  
  1. How to prepare for Erythrosin B (200964) 0,5%?

    1 answer
    1. Erythrosin B solutions can be prepared in various solvents. The dye is commonly prepared in water, buffered salt solutions or in ethanol. The Biological Stain Commission dissolves the dye in clove oil for the testing that is performed for certification of biological dyes. The solvent chosen will depend upon the staining applications. Erythrosin B can be dissolved in water to ~11 percent and 5% in ethanol. Some application recommend mixing or vortexing the solutions before use

      Helpful?

  2. Are there instructions for using Erythrosin B for fluorescent microscopy?

    1 answer
    1. Erythrosin B and Eosin B are considered synonyms; however, there are differences in their CAS numbers and CI numbers. Although there are no specific instructions for the use of product 200964, instructions are available for another Erythrosin B. This product can be used in a light microscopy procedure called hematoxylin and eosin staining. Additionally, both Erythrosin B and Eosin B are commonly used in H&E staining procedures and may be suitable for fluorescence microscopy for viability staining. While there is no product available for a staining procedure for viability staining, multiple procedures are available on the internet that can be considered for use with product 200964.

      Helpful?

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