推薦產品
尋找類似的產品？ 前往 產品比較指南
一般說明
MISSION pLKO.1-puro 对照载体是一种慢病毒质粒载体。该载体不含 shRNA 插入，在使用 MISSION shRNA 文库克隆的实验中用作阴性对照。 这让人们可以检测转染对基因表达的影响，并解读 shRNA 克隆看到的敲低效应。氨苄西林和嘌呤霉素抗生素抗性基因分别在细菌或哺乳动物细胞中提供选择。此外，通过与相容的包装质粒共转染，可在包装细胞 (HEK293T) 中产生自我失活复制无能的病毒颗粒。普尔科。1-puro 对照载体以 10μ 提供g 质粒 DNA，溶于 Tris-EDTA (TE) 缓冲液中，浓度为 500 ng/μl。
應用
MISSION® pLKO.1-puro 空载体对照质粒 DNA 已被用于合成和克隆 shRNA（靶向鼠分化簇 36 (CD36)），以生成慢病毒表达载体。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
法律資訊
使用本产品需遵守一个或多个许可协议。有关详细信息，请参阅http://sigmaaldrich.com/missionlicense。
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
客戶也查看了
Cardiospecific CD36 suppression by lentivirus-mediated RNA interference prevents cardiac hypertrophy and systolic dysfunction in high-fat-diet induced obese mice.
Zhang Y
Cardiovascular Diabetology, 14 (2015)
AMP-activated protein kinase (AMPK)-induced preconditioning in primary cortical neurons involves activation of MCL-1.
Anilkumar U
Journal of Neurochemistry, 124 (2013)
Gregory J Baker et al.
Cancer research, 74(18), 5079-5090 (2014-07-20)
Natural killer (NK) cells safeguard against early tumor formation by destroying transformed target cells in a process referred to as NK immune surveillance. However, the immune escape mechanisms used by malignant brain tumors to subvert this innate type of immune
Timothy Andrew Couttas et al.
Metabolites, 10(6) (2020-06-12)
The number, position, and configuration of double bonds in lipids affect membrane fluidity and the recruitment of signaling proteins. Studies on mammalian sphingolipids have focused on those with a saturated sphinganine or mono-unsaturated sphingosine long chain base. Using high-resolution liquid
Yu Fujita et al.
Cell reports, 24(12), 3296-3311 (2018-09-21)
Inflammatory bowel disease (IBD) is prevalent, but the mechanisms underlying disease development remain elusive. We identify a role for the E3 ubiquitin ligase RNF5 in IBD. Intestinal epithelial cells (IECs) express a high level of RNF5, while the colon of
文章
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
當使用慢病毒載體傳遞 shRNA 時，編碼 shRNA 的序列會整合到基因組中，而基因敲除的效果會傳給子細胞，繼續基因沉默。
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務