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重要文件

P8170

Sigma-Aldrich

Protein G−Peroxidase from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

同義詞:

Protein G−HRP from Streptococcus sp.

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46
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重組細胞

expressed in unspecified host

品質等級

共軛

peroxidase conjugate

形狀

lyophilized powder

技術

direct ELISA: 1:100,000 using human IgG

儲存溫度

−20°C

一般說明

Protein G is a bacterial cell wall protein, which has high affinity for immunoglobulin G (IgG). It is isolated from group G streptococcal strains.[1] Peroxidase from Streptococcus sp. is used to maintain the H2O2 homeostasis in cells. It aids the regulation of ABC Mn2+-permease complex (psaBCA) genes.[2]

特異性

The product binds IgG for most mammalian species (excluding feline) and can be used in ELISA or immunohistochemical formats. Protein was determined to be at 250-350ug per 250ug vial by UV absorbance. Reactive to chicken IgG as well.

應用

Protein G- Peroxidase from Streptococcus sp. has been used in indirect ELISA and immunoblotting.[3][4]

準備報告

Labeled with peroxidase type VI by a modification of the procedure of O′Sullivan, M.S., et al., FEBS Lett., 95, 311 (1978) which favors low molecular weight conjugates. Conjugate is purified by gel filtration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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條款

Reinheitszahl (RZ) is the ratio of absorbance due to hemin (A403, Soret region) to absorbance due to protein (A275).

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

建立以 2,2'-连氮基-双(3-乙基苯并噻唑啉-6-磺酸)为基质的过氧化物酶测定方法。

Questions

  1. After diluting this, how long does it keep in a fridge? (2-8 degrees Celsius)

    1 answer
    1. The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20°C.

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