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重要文件

EMS0005

Sigma-Aldrich

SOLu-Trypsin Dimethylated

Dimethlyated, Proteomics Grade, recombinant, expressed in Pichia pastoris, ready-to-use solution

同義詞:

Dimethylated Recombinant Trypsin for Protein Digestion in Mass Spectrometry, Dimethylated rTrypsin, Mass Spectrometry Trypsin Dimethlyated, No chemotryptic activity

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.78
暫時無法取得訂價和供貨情況

重組細胞

expressed in Pichia pastoris

品質等級

等級

Proteomics Grade

形狀

ready-to-use solution

效力

>10,000 units/mg

比活性

≥10,000 units/mg protein (Enzymatic activity)

適合性

suitable for mass spectrometry

UniProt登錄號

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

一般說明

SOLu-Trypsin Dimethylated (EMS0005) is our exclusive, Advanced Proteomics Grade enzyme that is solution stable for mass spectrometry. Designed to be stable in solution when refrigerated, SOLu-Trypsin Dimethylated can be used immediately without preparation. Other forms of trypsin require thawing or reconstitution, and must be discarded if not used immediately. SOLu-Trypsin Dimethylated allows excess product to be saved for future use, thus eliminating unnecessary waste and cost. SOLu-Trypsin Dimethylated is formulated with a high-purity recombinant trypsin, free of chymotryptic activity, to ensure high fidelity digestion.

特點和優勢

  • Ready to use - no preparation, such as reconstitution or thawing, is required
  • Fits seamlessly into established workflow - no need to modify protocols
  • Eliminates waste - remains stable in the refrigerator after use so there is no need to discard excess product
  • Recombinant, porcine sequence - no chymotryptic activity
  • Stable for short-term use at room temperature in an autosampler or on a liquid handling robot

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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文章

Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays

化学处理的重组胰蛋白酶在蛋白质组学和蛋白质表征分析中的应用评测

Questions

  1. What's the feature/benefit of EMS0005 over EMS0004? Thanks!

    1 answer
    1. The difference between SOLu-Trypsin Dimethylated (EMS0005) and SOLu-Trypsin (EMS0004) products is primarily the stability. The dimethylated form, EMS0005, is more resistant to autolysis over time.

      Please see the links below to review the datasheet for each product:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/535/ems0005bul.pdf
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/334/377/ems0004bul.pdf

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